基因传感器在环境微生物功能基因检测中的应用-微生物学报.PDF

微生物学通报 APR 20, 2008, 35(4): 565~571 Microbiology © 2008 by Institute of Microbiology, CAS tongbao@ 专论与综述 基因传感器在环境微生物功能基因检测中的应用 * 刘 灿 谢更新 汤 琳 黎媛萍 曾光明 ( 410082) 摘 要: 分子生物学的快速发展和人们对核酸的深入研究, 给基因传感器的研制和发展奠定了坚 实的基础。基因传感器是分子生物学与微电子学、电化学、光学等相结合的产物, 将在生命科学 与信息科学之间架起一座桥梁, 成为DNA 信息分析检测最有效的手段之一。在近年研究文献的基 础上, 评述了基因传感器的工作原理、分类及近年来国内外利用其进行环境微生物功能基因检测 等方面的研究现状, 并提出堆肥微生物功能基因检测将是基因传感器的一个重要发展方向。 关键词: 基因传感器, 微生物, 功能基因, 检测 The Application of Genosensor in the Detection of Functional Genes of Environmental Microorganisms LIU Can XIE Geng-Xin TANG Lin LI Yuan-Ping ZENG Guang-Ming (Department of Environmental Science and Engineering, Hunan University, Changsha 410082) Abstract: The fast development of the molecular biology and the further research on the nucleic acid set a solid foundation for the development of genosensor. Genosensor is the result of combining molecular biol- ogy with microelectronics, electrochemistry, optics and etc, which will build a bridge between the life sci- ence and the information science and become one of the most important technologies for DNA information analysis and detection. The working principle, classification of genosenor and the recent research on its ap- plication in the detection of functional genes of environmental microorganisms are discussed according to the latest literature. And it is pointed out that the application in the determination of microorganism func- tional genes in compost is an important development orientation of genosensor. Keywords: Genosensor, Microorganism,