鸡大肠埃希氏菌iss基因与其致病力相关性的研究-兽医学；预防兽医学专业论文.docxVIP

CoNT】烈TSCONTENTS CoNT】烈TS CONTENTS Abstract in Chinese ·I Abstract in English ·III 1 IntroductiOn ·1 1．1 Main biological characteristics ofEscherichia coli ··1 1．2 An overview of avian E．coli ··l 1．3 Pathogenic facmrs ofE．coli ．． j 1．3．1 Pili ”3 1．3．2 Iron transpoft system ’叶 1．3．3 Temperature sensitive hemagglutinin q 1．3．4 Toxin ·5 1．3．5 Outer membrane protein 一J 1．3．6 Serum resistance ”5 1．4 Research progress on／ss gene ofE．coli ·．o 1．5 Research purpose and meaning 7 2 Materials and methods 。o 2．1 The experimental materials o 2．1．1 Baterials ”8 2．1．2 Animals 8 2．1．3 Synthesis ofprimers ”o 2．1．4 The main reagent ’。 2．1．5 The main equipment ”。7 2．2 The experiment method y 2．2．1 Isolation and identification ofE．coli ·y 2．2．2 Nested PCR锄plification 1 0 2．2．3 Pathogenicity tests 1 2．2．4 Real time fluorescence quantitative PCR 。1 2．2．5 Serum resistance assay 一。 3 Results 一’ 3．1 Isolation and identification ofE．coli ”H 3．2 Pathogenicity tests detection of iss gene from E．coli by nested PCR ·15 3．3 Sequence alignment and evolutionary analysis ·1， 3．3．1 Different groups ofE．coli according to sequence 1 3．3．2 Evolutionary tree of iss gene ’‘1’ 3．3．3 AligIling and distances。f／ss gene 20 III 万方数据 东北农业大学农学硕士学位论文3．4 东北农业大学农学硕士学位论文 3．4 Pathogenicity tests detection ofiss gene from E．coli by nested PCR 23 3．5 Real time fluorescence quantitative PCR amplification of iss gene． ．．．．． ． ．． ． ．． ．． ．24 3．5．1 Standard of iss gene ． ．．24 3．5．2 Standard of GAPDH gene 25 3．5．3 The results ofqPCR ．26 3．6 Serum resistance tests ． ． ．．27 4 Discussion ．．29 4．1 The universality ofthe iss gene ofE．coli in different strains ．．29 4．2 Correlation between iss gene and pathogenicity ofE．coli analysis ．．30 4．3 Correlation between serum resistance and pathogenicity of E．coli analysis ．3 1 5 Conclusion ． ． ．．33 Acknowledgement ． ． ．．34 References ． ．36 Papers published in the period of Ph．M．education 42 IV