吉林省玉米丝黑穗病菌致病性分化与RAPD分析-植物病理学专业论文.docxVIP

林农业人学倾l。学位论文 林农业人学倾l。学位论文 吉林省玉米丝黑穗jlij菌致病忡分化‘，RAPD分析 摘要 本文采用鉴别寄主鉴定技术和基因组随机多态性扩增技术(RAPD)，研究了 吉林省玉米丝黑穗病菌存在致病性分化，并从遗传物质DHA水平证实病菌致病性 的分化并非是表型差异，而是一种基因的分化，具有较稳定的遗传物质基础。 经过大量的田问调查7Z．．K_T-接种鉴定，确定了登海1、吉单209、吉单180、 丹6 39、农大31 38、本育九、Mol 7和黄早四为较理想的玉米丝黑穗病鉴别寄主， 初步建立了吉林省玉米丝黑穗菌致病性分化的鉴别寄主体系，该鉴别体系中的6 个玉米品种及2个玉米自交系代表了吉林省正在推广使用的玉米品种资源和二 大骨干自交系，对今后鉴定和监测病菌致病类型的分化具有重要作用。 采用8个鉴别寄主将吉林省玉米丝黑稳病菌大致划分为3个致病类型。致病 类型A为强致病类型，来源于吉林、长春、公主岭和长岭，是玉米丝黑穗病发生 最严重的地区；致病类型c为弱致病类型，分布于白城地区；致病类型B其致病 性介于A、C类之间，主要分布于四平、通化、松原和延边地区。 玉米丝黑穗病菌致病性分化研究表明，鉴别寄主的鉴定结果和DNA遗传多态 №瞒助鉴定的结果关系密切，这进一步表明病原菌致病性的分化归根结底是控制 致病性的基因不同。采用鉴别寄主鉴定和DNA遗传多态性鉴定相结合的技术鉴定 体系来研究玉米丝黑穗病菌的致病性分化在国内外还属首次，本研究为进一步深 入研究玉米丝黑穗病菌的生理分化奠定了基础。 关键词： 玉米丝黑穗病菌：致病性分化：鉴别寄主：RAPD分析：吉林省 ．‘i林农业人学坝l’学位论文 ．‘i林农业人学坝l’学位论文 吉林省乐米丝黑穗摘菌致病h分化々RAPD分析 Abstract Pathogen collections ofSporisorium reilianum from Jilin，Changchun，Siping， Songyuan，Gongzhuling Baicheng，Tonghua，Changling et a1．were firstly studied by using identification on differential hosts and RAPD analysis in this paper． Through a great number of field investigations and inoculation identifications， six corn varieties(Denghail，Jidan209，Dan639，Nongda3138，Benyu9)and two corn inbred-lines(Mol 7 and Huangza04)widely popularized and used in Jilin province com production and corn breeding nOW were defined as ideal differential hosts for pathogens of Sporisorium reilianum．They represented different resistant and susceptible level of corn to Sporisorium reilianum and were able to group the pathogen collections of Sporisorium reilianum from different corn-grown areas in Jilin． Nine pathogen collections of Sporisorium reilianum were inoculated on 8 differential hosts in field．The results showed that 9 pathogen collections of Sporisorium reilianum were divided into 3 pathogenic types in which there were type A with hi曲pathogenicity,type B with moderate pathogenicity and C with weak pathogenicity．Type A were originated in most important com-grown areas including Changchun，Jilin，Gongzuling and Changling where corn head smut