加温联合冬凌草甲素对肝癌细胞株SMMC-7721作用的研究-外科学专业论文.docxVIP

PAGE PAGE 10 STUDIES ON SYNERGETIC EFFECTS ON HEPATOMA CELL LINE SMMC-7721 WITH HYPERTHERMIA AND ORIDONIN Abstract Objective: To investigate the effect of synergetic anti hepatocellular carcinoma of hyperthermia and oridonin, and the best combined pattern and the possible mechanisms of the combination of hyperthermia and oridonin ,which may provide useful evidence for clinical study. Methods: (1).The MTT was used to estimate the inhibition effect of hyperthermia and oridonin separately and together on cultured SMMC-7721 cell in vitre. To evaluate whether the combination of hyperthermia and oridonin. had a synergetic effect.(2).Apoptotic cells with Gimsa staining were observed under light microscope.(3).DNA contents of apoptotic cells and the apoptosis index were determined by flowcytometry. (4).AFP contents was determined by ELISA.(5).ALP、ALB contents were detected by biochemistry meter of the whole automatic movement. Results: (1).The use of hyperthermia and oridonin separately and together could remarkably inhibit growth of hepatomia cell, which showed dose-dependent and time-dependent effects. Oridonin is a drug without temperature threshold. The inhibition of HCC by usage of hyperthermia or oridonin separately could be significantly improved by usage of two together(p0.001). It also markedly improved in different combined pattern(p0.05).The inhibiting effect of simultaneous use of hyperthermia and oridonin on cancer cells was more than that of sequential of the two treatments(p0.05). (2). Hyperthermia and oridonin could result to apoptosis of cancer cell. The hoist of apoptosis rate was related to the increasing of temperature and dosage. Apoptosis index was higher in associated group than in that of hyperthermia or oridonin alone. (3).Using hyperthermia and /or oridonin could significantly reduce the value of AFP in culture supernate(p0.01). The effect of reducing the value of AFP in associated group was bigger than other groups(p0.01),but both had no signif