家蚕ssr标记和caps标记的筛选及其应用-动物学专业论文.docxVIP

家蚕徽卫星耨记耘CAPS标记的筛选及其应用品种的基因组I)NA的PCR多态分析。发现程2品种之间存在多态性的引物 家蚕徽卫星耨记耘CAPS标记的筛选及其应用 品种的基因组I)NA的PCR多态分析。发现程2品种之间存在多态性的引物 13对。其余的85对引物中条带清晰可进一步用于酶切多态分析的引物有 2S对。取其中16对弓l物终家蚕6令品转静PCR多态分毒嚣，在P50秘C108 之间有多态豹引物2对，在6个品种中其它晶种之间有多态的引物有2对。 在家骚全基因组序列公布以后，这将是寻找分子标记的最主要的方法之一。 关键诞家蚕徽墨震标记懿淤拣记多态遽转多铎牲 lI 摘螫羚甏V鏊瓢。巍鹱嚣N零A燃豁A挚P乳le笈毯oN 摘螫 羚甏V鏊瓢。巍鹱嚣N零A燃豁A挚P乳le笈毯oN o窘 MICRoS发rEbbITE M匕搬KERS AND CAPS MA嘲RS簟0 ANAl淄ZE G嚣N黔蕈lC D贰嘏鬏S差篁Y IN SlL薹(Wo歉M．嚣馥4撼YX l娃。疑I SHEN Li(Zoology)’ Directed by Professor HuangYongping Abstract In this pap％we constructed a 7 kb—insert genomic library from the silkworm strain‘P50’．The screening of the genomie library by the conventional hybridization method led to the isolation of38 mierosatellites harbouring clones。 Microsatellite polymorphism was assayed in 34 diverse silkworm strains。Of the 748 PCRs carried out，all but four produced amplified products of the expected size。22 microsatetIites were polymorphic。showing》i丢alleles per locus(mean 8．2、．The repeat length did not show any relationship with the degree of potymorphism in the present study．Heterozygosity ranged from 0．161—0．922 (mean§+74囝．PIC ranged from O，164固．922(mean 0，746)。The result of UPGMA analysis is up to the geographica／distribution of silkworm．Hence， micmsatellites,ranked according to their information content，are recommended aS markers of choice for silkworm fingerprinting and suggestions瓣provided for interpreting band profiles and the correct sizing ofalleles， A silkgland DNA extraction method WaS developed for rapid identification of III 家委擞卫星橱记鞋CAPS檬记黔簿逸艘其应孀silD,vorm 家委擞卫星橱记鞋CAPS檬记黔簿逸艘其应孀 silD,vorm genotype．The method was applied to 6 silkworm strains．This method wilI be useful for genotypic selection that requires rapid screening of large populations by PCR and AFLP analysis。The meNod includes tWO major steps({) grinding of a pair of silkglands谢t11 a roller in 1．5 ml tube and then treatment by proteinase K in an SDS exuaction buffer，and(ii)precipitating protein with NaAC+About 200垫g of D