北大生物化学完整课件chapter4-1.pptxVIP

The Three-Dimensional Structures of Proteins 蛋白质的三维结构 ; 分离氨基酸常用的是带有耐酸性非常强的磺酸根SO3－Na＋（以盐的形式出现）的强阳离子交换树脂。首先将这种树脂填充到柱子中，然后注入含有样品的流动相，样品中含有阳离子成分X＋，通过静电吸引，与树脂中的带电基团相互作用，结果X＋与Na＋交换，即发生阳离子交换后，形成SO3－X＋。; Ion-exchange Chromatography 离子交换;;A protein Crystal Is not a rock or salt.;Some protein crystals;The use of recombinant proteins 重组蛋白;; “Perhaps the most remarkable features of the molecule are its complexity and its lack of symmetry. The arrangement seems to be almost totally lacking in kind of regularities which on instinctively anticipates, and it is more complicated than has been pre