近红外透反射光谱法测定牛奶成分.PDF

※分析检测 食品科学 2013, Vol.34, No.20 153 近红外透反射光谱法测定牛奶成分 杨晋辉，卜登攀，王加启*，周凌云，马 露，张军民 ( 中国农业科学院北京畜牧兽医研究所，动物营养学国家重点实验室，北京 100193) 摘  要： 150 为探索近红外透反射光谱法检测牛奶中主要成分的可行性，从牧场采集 份乳样，并用乳质分析仪测定 其中的蛋白质、脂肪、乳糖、总固形物、非脂固形物含量。通过光谱数据结合偏最小二乘法以及完全交互验证建 立回归模型。结果表明：中长波(1300～2500nm)对乳成分的模型贡献较大；蛋白质、脂肪、乳糖、总固形物、非 0.96 0.90 0.90 0.91 0.92 4.97 3.19 3.15 脂固形物模型的验证集决定系数分别达到 、 、 、 、 ，相对分析误差分别为 、 、 、 3.37 3.54 10 、 ；对 个未知样品的预测结果表明，该模型对蛋白质、乳糖和非脂固形物含量的预测效果较好，相对误 差均小于1.40%。 关键词：牛奶；近红外透反射光谱；乳成分；偏最小二乘法 Determination of Chemical Composition of Milk Using Near-Infrared Reflectance Spectroscopy YANG Jin-hui ，BU Deng-pan ，WANG Jia-qi*，ZHOU Ling-yun ，MA Lu ，ZHANG Jun-min (State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China) Abstract ：This study reports on the application of near infrared reflectance spectroscopy to determine the major chemi- cal components in milk. Totally 150 milk samples were collected from the same dairy farm, and the contents of protein, fat, lactose, total solids and non-fat solids in milk were measured. Based on the spectral data, a regression model for each com- ponent was proposed by partial least square regression combined with cross-validation. The results showed that middle- and long- wavelength (1300–2500 nm) absorption contributed much to the regression models. The coefficient of determination for the models for protein, fat, lactose, total solids and non-fat solids in validation sets were 0.96, 0.90, 0.90, 0.91 and 0.92, respectively, with relative errors of 4.97, 3.19, 3.15, 3.37 and 3.54,