产油脂耐盐真菌Salicorn5的分离鉴定与发酵产物.pdf

摇 南京农业大学学报摇 2013,36(5):99-107 http:/ / 摇 Journal of NanjingAgricultural University doi:10.7685/ j.issn.1000-2030.2013.05.017 試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試試 王惠,孟晓露,赵育卉,等.产油脂耐盐真菌Salicorn5 的分离鉴定与发酵产物表征[J].南京农业大学学报,2013,36(5):99-107 产油脂耐盐真菌Salicorn5 的分离鉴定与发酵产物表征 王惠,孟晓露,赵育卉,刘天行,辛志宏* (南京农业大学食品科技学院,江苏 南京210095) 摘要:对分离自盐生海芦笋的1株代号为Salicorn5 的耐盐真菌进行鉴定,提取总DNA后分别PCR扩增18SrDNA和ITS1- 5.8S-ITS2 区域,测序并进行同源性分析,构建 ITS基因系统发育树,同时结合形态学分析,将该菌鉴定为小克银汉霉属 (Cunninghamella sp.)。 该菌经静置发酵后,用环己烷提取发酵产物,并用硅胶柱层析分离得到3个油脂单体化合物,化合 物经核磁共振(NMR)和质谱(MS)解析为油酸、亚油酸和1,3油酸双甘油酯。 该研究结果说明,小克银汉霉属Salicorn5菌 株能够高产不饱和油脂,具有潜在的工业化应用价值。 关键词:海芦笋;内生真菌;分离鉴定;不饱和油脂;结构解析 中图分类号:TS255.1摇 摇 摇 摇 文献标志码:A摇 摇 摇 摇 文章编号:1000-2030(2013)05-0099-09 Isolation and identification of an oil鄄producing halotolerant fungus Salicorn5 and characterization of the fermentation products WANG Hui,MENG Xiaolu,ZHAO Yuhui,LIU Tianxing,XIN Zhihong* (College of Food Science and Technology,Nanjing Agricultural University,Nanjing210095,China) Abstract:In this study,a halotolerant fungus Salicorn5 isolatedfromthe halophyte Salicornia bigelovii was identified.18S rDNA and ITS1鄄5.8S鄄ITS2 region were amplified and sequenced respectively after the extraction of genomic DNA. Homology analysis was conducted and phylogenic tree based on ITS gene sequence was constructed.Combined with morphological analysis,the strain was identified as Cunninghamella species.Thefermentation products of this strainwere extractedusing cyclohexane after fermentation,and three compoundswere obtained by silica gel column chromatography.Their structures were elucidated as oleic acid,linoleic acid and 1,3 oleic acid diglyceride on the base of nuclear