干酪乳杆菌LC2W胞外多糖提取工艺研究.pdfVIP

( ) 华北农学报 ·2 0 0 8 , 2 3 增刊 : 1 18 12 1 干酪乳杆菌 L C2W 胞外多糖提取工艺研究 1 2 1 1 1 ,2 刘翠平 ,叶　蕾 ,吴正钧 ,王荫榆 ,郭本恒 ( 1 光明乳业股份有限公司技术中心 ,上海 　200072 ;2 上海水产大学 食品学院 ,上海 　200090) 　　摘要 :对影响干酪乳杆菌LC2W 胞外多糖提取的主要工艺参数进行了研究 。发酵液经超滤浓缩 ,三氯乙酸脱蛋 白,乙醇沉淀等处理 ,得到粗多糖 。单因素和正交试验结果表明 ,用截留分子量为 10 kDa 的超滤膜将发酵上清液浓缩 4 倍后 ,用终浓度 6 %的三氯乙酸脱蛋白,再用 5 倍体积 95 %乙醇4 ℃沉淀24 h ,多糖提取效果较佳 。在此条件下 ,蛋白 脱除率可达 8835 % ,EPS 提取率可达 9217 % 。 关键词 :干酪乳杆菌 ;胞外多糖 ;提取 ( ) 中图分类号 :TS24 　　文献标识码 :A 　　文章编号 :1000 - 7091 2008 增刊 - 0118 - 04 Optimization of Preparation Procedures for Exopolysaccharides from L actobacillus casei L C2W L IU Cuiping1 ,YE Lei2 ,WU Zhengj un1 ,WAN G Yinyu1 , GUO Benheng1 ,2 ( 1Technical Center of Bright Dairy and Food Co .Ltd . ,Shanghai 　200072 ,China ; 2College of Food Science and Technology ,Shanghai Fisheries University ,Shanghai 　200090 ,China) Abstract :The parameters involved in preparation of exopolysaccharides ( EPS) from L actobacillus casei LC2W were studied. Supernatant fermented MRS broth by L b. casei LC2W containing EPS was firstly concentrated by ultrafiltration , then proteins in the retentate were eliminated by addition of trichlorocetic acid ,and finally exopolysaccharides were pre cipitated by alcohol . Results of single factor test and orthogonal experiments showed the optimized EPS preparation proce dure was as the following :the supernantant of cultured MRS broth was firstly ultrafiltrated to 1/ 4 of original volume by us ing MWCO of 10 kDa membrane ,the proteins in the retentate was eliminated by TCA at an ultimate concentra