牙体脱钙法和劈牙取髓法观察牙髓组织中VEGF表达的效果.pdfVIP

20 5 ( ) Vol. 20 No. 5 2010 9 Journal of Jiangsu University(M edicine Edition) Se t. 2010 VEGF 关为群, 王清妹 (, 350001) [ ] : ( vascu lar endothelial grow th factor, VEGF) , : 30 , , VEGF , : , , VEGF ; , , : , [] ; ; [] R780. 2 [] A [] 1671- 7783( 2010) 05- 0408- 04 Comparison of decalcificationand splitting teeth inVEGF immunohistochemicalstudy of human dentalpulp G UAN W ei-qun, WANG Q ing-m ei ( De artm ent of Stom atology, the A ffiliatedU nion Hos ital of FujianM edicalUniversity, Fuzhou Fujian 350001, China) [Abstract] Objective: To com are mi munohistochem ical staining results of vascular endothelial grow th factor (VEGF) in human dental ul by decalcification and s litting teeth and ex lore the a lication of de- calcification in mi munohistochem ical study of human dental ul . ethods: Th irty healthy and mature mand ibu lar remolarsw ere collected for orthodontic reasons and stud ied w ith VEGF mi munohistochem istry stain ing res ectively by decalcification and s litting teeth, roduced tw o d ifferent dental ul sections and to observe the stain ing resu lts betw een the tw o methods under the m icrosco e. Results: Sections rocessed w ith decalcification workedw el,l in wh ich ul cells, odonoblasts and dentinal tubu le structure w ere clear andVEGF ositive distribution w ere w ell defined; W hile, sections dealt w ith s litting teeth w ere w ith in- com lete odonoblasts, oor staining and weak contrast. Conclusion: Decalcification a licated in dental