痛风颗粒抗痛风有效部位群研究.pdfVIP

• 590 • 中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 5 期 2013 年 3 月 痛风颗粒抗痛风有效部位群研究 * 刘 静，徐玲玲 ，徐 熠 上海中医药大学附属岳阳中西医结合医院 药剂科，上海 200437 摘 要：目的 研究痛风颗粒不同提取部位（群）的抗炎、降尿酸作用及对肾脏的影响，筛选痛风颗粒抗痛风的有效部位（群）。 方法 大鼠足跖注射微晶型尿酸钠制备踝关节肿胀、足跖肿胀模型，腺嘌呤＋盐酸乙胺丁醇制备大鼠高尿酸血症模型。通过 检测大鼠足肿胀率、血尿酸（UA ）值、肌酐（Cr ）、血尿素氮（BUN ）水平和肾脏系数，筛选痛风颗粒不同提取部位中的 有效部位（群）。结果 痛风颗粒 A 部位（主要由黄酮、生物碱、有机酸组成）显示出显著的抗炎、降尿酸和一定的肾脏保 护作用：显著抑制尿酸钠致大鼠的足跖肿胀及踝关节肿胀（P ＜0.01 ）；显著降低高尿酸血症大鼠的血 UA 、Cr 及 BUN 水平 （P ＜0.01 ）；但与模型组比较，各给药组肾脏系数无显著改变。结论 在各提取部位中，A 部位降尿酸、抗炎作用最强，与 痛风颗粒全方作用强度相当。 关键词：痛风颗粒；痛风；有效部位群；尿酸；抗炎 中图分类号：R285.5 ；R971.1 文献标志码：A 文章编号：0253 - 2670(2013)05 - 0590 - 05 DOI: 10.7501/j.issn.0253-2670.2013.05.018 Antigout active fractions in Tongfeng Granule LIU Jing, XU Ling-ling, XU Yi Department of Pharmacy, Yueyang Hospital of Integration of Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China Abstract: Objective To observe the therapeutic effect of the various active fractions in Tongfeng Granule on gouty arthritis, gouty hyperuricemia with renal protection. Methods The swelling models of ankle and voix pedis in rats were induced by microcrystal monosodium urate (MSU) and the hyperuricemia model in rats was induced by adenine and ethambutol. The swelling rate, blood uric acid (UA) level, creatinine (Cr), blood urea nitrogen (BUN) level, and kidney indexes of rats were measured to screen antigout active fractions in Tongfeng Granule. Results Fraction A (mainly consisting of flavonoids, alkaloids, and organic acids) in Tongfeng Granule showed the significant effect on gouty arthritis, gouty hyperuricemia with certain renal protection; It could significantly reduce the foot swelling rate (P 0.01) and the contents of UA, Cr, and BUN in rats (P 0.01); But compared with the model group, there was no significant