重力流排水管道内流态对生物膜菌落结构的影响.pdfVIP

第 11卷　 第5 期 环 境 工 程 学 报 Vol. 11,No.5 2 0 1 7 年 5 月 Chinese Journal of Environmental Engineering May 2 0 1 7 重力流排水管道内流态对生物膜菌落结构的 影响 ∗ 艾海男 ,张青,何强,徐警卫,王银亮 重庆大学,三峡库区生态环境教育部重点实验室,重庆 400045 摘　 要　 为探究排水管内壁面剪切力与生物菌落结构之间的关系,基于排水管道内流态与壁面剪切应力二维回归模型,建 立了3套管道生物膜培养装置,利用微电级技术获得了3 种不同剪切力条件下生物膜厚度随时间变化规律,结果显示:剪 切力分别为 1.12、1.29 和 1.45 Pa 时,对应生物膜稳定厚度分别为(2.40 ±0.15)、(2.70 ±0.15)和(2.20 ±0.15)mm。 结合 高通量测试技术与溶解氧微电级技术研究了不同壁面剪切力对生物膜菌落结构的影响,得出F =1.29 Pa 为异养菌最适生 长的剪切力,有利于 COD 的去除,COD去除率达到40.13%。 关键词　 排水管道;管壁剪切应力;生物膜;O ;菌落结构2 中图分类号　 X703　　 文献标识码　 A　 　　 文章编号　 1673-9108(2017)05-2845-06　　 DOI　 10.12030/ j.cjee.201606106 Impact of flow pattern of drainage pipe on structure of biofilm colonies ∗ AI Hainan ,ZHANG Qing,HE Qiang,XU Jingwei,WANG Yinliang Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment,Ministry of Education,Chongqing University,Chongqing400045,China Abstract　 In order to explore the relationship between the shear stress in drainage pipes and the structure of mi- crobial colonies,three devices were built to cultivate pipe biofilms. This approach is based on previous research involving a two dimensional regression model used to relate wall shear-stress and flow patterns in drainage pipes. The variation of biofilm thicknesswith time under three different shear-stress conditionswas obtained using micro electrode technology. The results showed that when shear stress was 1.12,1.29,and 1.45 Pa,the stable thick- ness of the respective biofilm was (2.40 ±0.15),(2.70 ±0.15),and (2.20 ±0.15)mm. High-throughput tes- ting and dissolved-oxygen micro-electrode techniqueswere used to study the influence of differences in wall shear stress on the structure of the biofilm colonies. Wefound that F =1.29 Pawasthe optimum shear stressfor growth of heterotrophic bacteria beneficial for the removal of COD. Under this condition,the removal rate reached 40.