东北红豆杉化学成分的分离与鉴定-药学学报.DOC

· PAGE 1400· 药学学报 Acta Pharmaceutica Sinica 2010, 45 (11): 1398?1401 吴 迪等: 东北红豆杉化学成分的分离与鉴定 · PAGE 1401· · PAGE 1398· 药学学报 Acta Pharmaceutica Sinica 2010, 45 (11): 1398?1401 东北红豆杉化学成分的分离与鉴定 吴 迪1, 李亚男2, 3, 吴立军2, 3, 高慧媛2, 3* (1. 辽宁省公安厅刑科所, 辽宁 沈阳 110032; 2. 沈阳药科大学中药学院, 辽宁 沈阳 110016; 3. 沈阳药科大学基于靶点的药物设计与研究教育部重点实验室, 辽宁 沈阳 110016) 摘要: 利用硅胶柱色谱、制备高效液相色谱等分离方法, 从东北红豆杉提取物中分离得到3个化合物。根据理化性质和波谱数据对化合物进行结构鉴定, 分别鉴定为 (E)-1-methoxy-2-O-(p-coumaroyl)-myo-inositol (1)、2- deacetoxy-7β, 9α, 10β-trideacetyltaxinine J (2) 和 (3aS, 4aR, 6S, 8S, 8aS, 9R, 10R, 10aS)-benz[f]azulene-6, 8, 9, 10 (3H)-terol, 3a, 4, 4a, 5, 6, 7, 8, 8a, 9, 10-decahydro-10a-(1-hydroxyl-1-methylethyl)-1, 8a-dimethyl-5-methylene (3)。其中化合物1为一新化合物, 化合物2、3为两个新天然产物。 关键词: 东北红豆杉; 化学成分; (E)-1-methoxy-2-O-(p-coumaroyl)-myo-inositol 中图分类号: R284.2 文献标识码: A 文章编号: 0513-4870 (2010) 11-1398-04 Isolation and identification of chemical constituents from Taxus cuspidta Sibe et Zucc WU Di1, LI Ya-nan2, 3, WU Li-jun2, 3, GAO Hui-yuan2, 3* (1. The Liaoning Provincial Public Security Office, Shenyang 110032, China; 2. School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China; 3. Key Laboratory of Structure-Based Drug Design＆Discovery, Ministry of Education, Shenyang Pharmaceutical University, Shenyang 110016, China) Abstract: Three compounds were isolated from the extract of Taxus cuspidta Sibe et Zucc with the column chromatography on silica gel and preparative HPLC methods. Their structures were identified according to the physicochemical properties and spectral analysis, and they were identified as (E)-1-methoxy-2-O-(p-coumaroyl)- myo-inositol (1), 2-deacetoxy-7β, 9α, 10β-trideacetyltaxinine J (2) and (3aS, 4aR, 6S, 8S, 8aS, 9R, 10R, 10aS)- benz[f]azulene-6, 8, 9, 10 (3H)-terol, 3a, 4, 4a, 5, 6, 7, 8, 8a, 9, 10-decahydro-10a-(1-hydroxyl-1-methylethyl)-1, 8a-dimethyl-5-methylene (3). Among them, compound 1 was a new compound, and compounds 2, 3 were two novel natural p