HCV变异血清学检测技术初步研究及小样本临床评价.docVIP

中国药品生物制品检定所硕士论文 英文摘要 conformation polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) and so on, through the analysis of HVR1 genetic complexity, reflecting the whole HCV genome variation. However, these methods can not be separated HCV-RNA extraction - RT-PCR three links, and the methods need long time, high cost, difficult to promote clinical. In this study, we take the largest variation of HCV HVR1 as the target for the study, using bioinformatics tools, parallel to the thousands of articles HVR1 peptide antigen and the simulation, a representative mutant HCV HVR1 antigen c