除草剂与乙酰乳酸合成酶相互作用的分子机理研究进展_姜玲.pdfVIP

2007, 9( 1): 06-13 Ch inese Journal of Pesticide Science 专论与综述 姜 玲, 王相晶, 向文胜* ( , 1 0030) : 综述了酵母乙酰乳酸合成酶( ScALS)与磺酰脲类除草剂氯嘧磺隆( chlormi uron-ethy,l CE) 形成的复合物在 0. 28 nm 分辨率下的 体结构及拟南芥乙酰乳酸合成酶( A tALS)与磺酰脲类和咪 唑啉酮类除草剂复合物的三维结构除草剂的分子结构与酶底物并不相似, 但它们与酶形成的复 合物可阻断底物进入酶活性位点通路而起抑制作用连接磺酰脲的 10 个氨基酸残基同样连接咪 唑喹啉酸, 另有 6 个残基只与磺酰脲而不与咪唑喹啉酸相连, 有 2 个残基只与咪唑喹啉酸而不与磺 酰脲相连, 即两种抑制剂占据了特别的重叠位点, 但以不同方式连接抗性杂草的产生是因为突变 株ALS的残基位点变异, 从而引起除草剂与ALS结合方式的变化这些研究对进一步理解除草剂 与靶分子的作用方式及除草剂的分子药物设计具有重要的指导作用 : 乙酰乳酸合成酶; 磺酰脲类除草剂; 咪唑啉酮类除草剂; 分子机理 : Q71 : A : 1008-7303( 2007) 01-0006-08 Advances inM olecularM echan ism of Interaction betw een H erbicide and A cetolactate Synthase * JIANG Ling, WANG X iang-jing, XIANG W en-sheng (D epartm ent of Life Science Academ y, Northeast Agricu ltu re University, Harbin 1 0030, Ch ina ) Abstract: The 0. 28 nm resolution crystal structure of yeast acetolactate synthase in complex w ith a su lfonylurea herbicide, chlormi uron ethyl and the 3D structure of A rab idop s is thaliana acetolactate synthase in complex w ith su lfonylureas and mi idazolinone herb icidesw ere review ed. N either class of these molecu les has a structure thatmay m mi ic the substrates for the enzyme, they act by block ing a channel of theALS throughw hich access to the active site. Ten of the am ino acid residues that bind the sulfonylureas can also b ind w ith mi azaquin ( IQ). Six add itional residues interact only w ith the sulfonylureas, whereas there w ere tw o residues that b ind IQ but not the su lfonylureas. Themutation ofALS residue sites causes the appearance of resistant w eed, and it also changes the b inding mode bewt een herb