十字花科蔬菜种传黑斑病.pdfVIP

2003, 8( 5) : 61~ 68 Journal of China Agricultural Universit 1 2 1 肖长坤 李勇 李健强 ( 1. , 100094; 2. , 65211) 黑斑病是危害十字花科蔬菜生产的世界性真菌病害, 20 世纪90 年代前后在我国北方严 发生, 目前在生 产中仍有潜在风险黑斑病病原芸薹链格孢甘蓝链格孢萝卜链格孢的鉴定主要借助病菌形态病原变异核酸 序列同工酶与酶联免疫分析, 其中从核酸水平进行病原鉴定更为快捷; 黑斑病菌毒素等次生代谢产物是 要致病 因子分子生物学方法已用于十字花科蔬菜种传黑斑病菌种的检测和鉴定在该病害的防治方法中, 种子处理及 施用高效低毒杀菌剂是有效途径今后应在分子水平上注 对病原种群构成病害流行及种子健康检测方法的研 究, 同时加强该病害生物防治的基础和技术性探索 十字花科蔬菜; 种传黑斑病; 芸薹链格孢; 甘蓝链格孢; 萝卜链格孢 S 43511 2003) A Research in seedborne black spot disease in cruciferous vegetables 1 2 1 Xiao Changkun , Li Yong , Li Jianqiang ( 1. College of Agronomy and Biotechnology, China Agricultural Uni ersity, Beijing 100094, China ; 2. Department of Food Science, Uni ersity of MissouriColumbia, MO 65211, USA) Abstract Black leaf spot is a worldwide fungal disease of crucifers important. It caused se ere losses in North China during the 1990s and is still a potential threat at present. Alternaria brassicae, A. brassicicola and A. japonica are pathogens of black leaf spot. The pathogenic fungus can be identified by their morphology, ariation, nucleotide acid sequence, isoenzyme, and ELISA. Among these techniques, the nucleotide acid sequence analysis is No. 1 much quick and practical. Toxins produced by the fungus are significant pathogenic factors. Molecular biological methods ha e been used for detection and identification of the pathogens in seeds. Effecti e measures for the disease control include seed treatment and fungicides of lower toxicity. It is proposed that future directions of in estigating should be the pathogenic constituents, disease epidemic, and methods for seed health testing on the molecular le els, as well