乳腺癌中p73α和p53表达与化疗药物敏感性的关系.docxVIP

乳腺癌中p73α和p53表达与化疗药物敏感性的关系 【关键词】 乳腺肿瘤  Relationship between expression of p73α and p5and chemosensitivity in breast cancer  【Abstract】 AIM: To investigate the relationship between the expression of p73α and p5in breast cancer cells and chemosensitivity. METHODS: Twelve surgical samples of pathologically diagnosed breast cancer were used. The cancer cells were isolated and cultured in the incubator at7℃,0 mL/L CO2 in vitro. The inhibitory rates of cancer cells by kinds of anticancer drugs (EPI, MMC,FU and DDP) were assayed by MTT method. Immunocytochemistry was used to detect the expression of p73α and p5in the cancer cells. RESULTS: The sensitivity of the 1breast cancer cells in ×PPC withinh in vitro was2% (MMC),% (EPI) and 17% (bothFU and DDP). The inhibitory rates of MMC, EPI,FU and DDP were significantly higher in p73α positive cancer cells than in p73α negative cancer cells. A positive correlation was found between the expression of p73α and chemosensitivity in all the four anticancer drugs. CONCLUSION: The expression of p73α is related to the chemosensitivity of the breast cancer cells and it can be used as one of the markers for clinical assessment of the effect of chemotherapy.  【Keywords】 breast neoplasms; genes, p73α; genes, p53; chemosensitivity  【摘要】 目的： 研究 乳腺癌细胞p73α、p53表达与化疗药物敏感性的关系. 方法 ： 选取病理诊断为乳腺癌的手术切除标本12例，体外分离细胞作培养， 应用 四甲基偶氮唑盐法 分析 表阿霉素、丝裂霉素、5氟脲嘧啶、顺铂4种化疗药物对乳腺癌细胞的相对抑制率，通过免疫细胞化学染色法分析乳腺癌细胞p73α、p53的表达. 结果： ×PPC浓度时乳腺癌细胞对4种化疗药物的敏感率分别为MMC、EPI、5Fu、DDP；p73α的乳腺癌细胞对4种化疗药物敏感性均高于p73α的乳腺癌细胞，p73α阳性表达程度与乳腺癌细胞对EPI、MMC、5Fu、DDP4种化疗药物的敏感性存在正相关关系. 结论： p73α的表达与乳腺癌细胞对化疗药物敏感性有关. 　　【关键词】 乳腺肿瘤；基因，p73α；基因，p53；化疗敏感性 0引言  　　乳腺癌临床化疗有效者能显著延长生存期，对化疗药物的抵抗被认为是乳腺癌化疗失败的原因之一. 目前 对乳腺癌的化疗多是依据临床经验选择相应化疗方案，使 治疗 存在盲目性，因此有必要寻找一些能对乳腺癌化疗疗效起预测作用的指标，以指导临床化疗提高疗效. 我们采用体外培养的人乳腺癌细胞，应用MTT法及免疫细胞化学染色法，研究p73α, p53表达与乳腺癌细胞对化疗药物敏感性的关系如下. 1材料和方法  材料  我院经病理诊断为乳腺癌，术前未经放疗、化疗的手术切除的新鲜癌组织12例，放入不含小牛血清的RPMI1640培养液中迅速送往实验室. 将表阿霉素(EPI)、丝裂霉素(MMC)、5氟脲嘧啶(5FU)、顺铂(DDP)分别用生理盐