三氧化二砷对小鼠骨髓细胞抗氧化能力及增殖活性的影响-临床兽医学专业论文.docxVIP

华中农业大学2006届硕士学位论文E髓ct 华中农业大学2006届硕士学位论文 E髓ct of Arsenic Trioxide on Antioxidant Abilities and Proliferative Activities of Marrow Cells in Rats Abstract Arsenic，which is a higllly poisonous metalloid,causes acute and chronic adverse health effects，including skin lesions，hyperkeratosis,blackfoot disease and cancer。m exact mechanism of the action of arsenical toxicity is not known clearly，but several hypotheses have been proposed．Oxidative stress，altered cell proliferation,genotoxicity including altered DNA methylation,CO-carcinogenesis,and tumor promotion are the potendalⅡ幢dⅢ锄s．described previously．Oxidative damage has been found to play锄1 important role in the arsenic-induced toxicity and earcinogenicity of many organisms． Recently,organic arsenic haz bean widely used in livestocks production,and arsenic trioxide has been successfully employed in the treatments of the majority of Ca$eS of acute promyeloeytie leukemia(APL)．A better understanding of the action of arsenic on marrow cells will make a mole confident assessment ofthe risks associated with arsenical therapy and arsenical exposure．With the purpose ofpreventing drug abuse and protecting human health，we observed the effect ofAS203 on antioxidant abilities and proliferative activities ofmarrow ceils in vivo and vitro． In the first experiment,fifIy rats were separated randomly into five groups of ten rats each,and were respectively administered via ig the water containing arsenic(0，5，20， 80 and 320 mg／L)for tllree months．Then the antioxidam abilities of marrow cells。 hemanalysis parameters of peripheral blood and viscera coefficients of organs were analyzed after rats werc killed．In the second experiment,marrow cells were collected from healthy rats to determine the effect of arsenic on proliferative abilities of marrow cells by MTT method． n地results of the first experiment were as follows：(1)Compared with the control group。the activities of GSH．Px and SOD as well as the content of GSH in rat marrow cells incre