武汉大学《分子生物学》期末考试试题.doc

武汉大学生命科学学院 2003－2004学年第一学期期末考试 《分子生物学》试卷 Final exam of Molecular Biology Course (Spring 2004) 年级______ 专业________ 学号_________ 姓名_______成绩______ PART I: DESCRIPTION (2 points each) Your answer should describe what each item is and how it functions in the cell. Diagrams, structure and sequence information should be included in your answer, as necessary. Yeast artificial chromosome RNA interference Proteomics Shine-Dalgarno sequence Alternative splicing Ribozyme ?-dependent termination RNA editing DNA lesions Protein targeting PART II: MULTIPLE CHOICES (1 points each) Select the one best answer for each question. 1. The catalytic activity for peptide bond formation (the peptidyl transferase activity) is located in the: RNA of the large ribosomal subunit. leader sequence of the messenger RNA. RNA of the small ribosomal subunit. proteins of the small ribosomal subunit. proteins of the large ribosomal subunit. 2. Bidirectional and semi-conservative are two terms that refer to: transcription. translation. replication. all of the above. none of the above. 3. The fact that most amino acids are specified by multiple codons is known as: the “wobble” phenomenon. the universality of the genetic code. codon bias. the anticodon hypothesis. the redundancy of the genetic code. 4. RNA polymerase I is the eukaryotic enzyme responsible for: transcription of ribosomal RNA. transcription of transfer RNA and other small RNA species. transcription of messenger RNA. initiation of Okazaki fragment synthesis in DNA replication. 5. Restriction enzymes can cleave DNA that is either single-stranded or double-stranded, as long as it contains the appropriate recognition site. 1) True 2) False 6. Information about the sequence of the coding region of a gene is best obtained from: a YAC clone. a genomic clone. a cDNA clone. the protein. 7. A chromatography method that can be used specifically to purify proteins based on their charge is: gel filtration chromatography. ion-exchange chromatography