水杨酸对高温强光胁迫下小麦叶片蛋白激酶和磷酸酯酶-河南农业科学.pdf

河南农业科学, 2011,40(4) :4548 Journal of Henan Agricultural Sciences 1,2 3 3 1 1 1 李利红 , 邢晓丽 , 陈 红, 赵会杰, 梁书荣, 曲小菲 ( 1. , 450002; 2. , 450011; 3. , 450000) : 为了探讨外源水杨酸对高温强光胁迫下小麦叶片蛋白激酶和磷酸酯酶活性的 节作用,在 灌浆期对小麦叶面喷施0.5mmol/ L 水杨酸后, 进行高温强光胁迫处理, 并测定蛋白激酶活性和磷 酸酯酶活性同时用 1mmol/ L 蛋白激酶抑制剂(FS A) 处理进行验证结果表明, 外源水杨酸处 理显著提高了2 种酶活性,蛋白激酶相对活性最高达312%, 磷酸酯酶相对活性最高达217%外源 水杨酸通过促进高温强光胁迫下小麦叶绿体蛋白的可逆磷酸化反应,保护了光合机构的完整性 : 小麦; 水杨酸; 蛋白激酶; 磷酸酯酶; 高温强光胁迫 : S512. 1 : A :2011) Regulation of Exogenous Salicylic Acid to the Activity of Protein Kinases and Phosphatase in Wheat Leaves under Heat and High Irradiance Stress 1, 2 3 3 1 1 1 LI Lihong ,XING Xiaoli , CHEN Hong ,ZH AO Huijie , LIANG Shurong , QU Xiaofei ( 1.College of Life Sciences, Henan Agricultural U niversity,Zhengzhou 450002,China; 2. Department of Pharmaceutical Engineering, Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China; 3. Workstation of Soil and Fertilizer, Agriculture ureau of Zhongmu County, Zhongmu 450000,China) Abstract: Effects of exogenous SA on the activity of protein kinases and phosphatase in wheat ( Tr i ticum asetiv um L.) leaves at filling stage were studied under heat and high irradiance stress. 0.5mmol/ L SA was sprout to wheat leaves at filling stage, and then the wheat plants were put under heat and high irradiance stress.T he activity of protein kinases and phosphatasewere deter mined. 1mmol/ L FS A was used at the same time to validate the effects of SA. T he results showed that SA increased the activity of protein kinases to 312% and that of phosphatase to 217% . Therefore, exog