炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学-物理化学学报.pdf

物理化学学报(Wuli Huaxue Xuebao) December Acta Phys. -Chim. Sin. 2012, 28 (12), 2867-2873 2867 [Article] doi: 10.3866/PKU.WHXB201209201 炭气凝胶纳米颗粒固定葡萄糖氧化酶的直接电化学 于志辉1 宿婷婷1 任翠华1 李 钒1 夏定国2,* 程水源1 1 2 ( 北京工业大学环境与能源工程学院, 北京100124; 北京大学工学院, 北京100871) 摘要: 利用间苯二酚和甲醛在碱性环境下制备炭气凝胶(CA), 通过扫描电镜(SEM)、透射电镜(TEM)、比表面 积测试Brunauer-Emmett-Teller (BET)等方法分析载体的形貌结构; 以CA 为载体通过吸附法固定葡萄糖氧化 酶(GOD)并修饰玻碳(GC) 电极, 得到GOD/CA/GC 电极. 在0.1 mol ·L-1 磷酸盐缓冲溶液中, 利用循环伏安法研 究了GOD/CA/GC 电极的直接电化学行为和对葡萄糖的催化性能. 结果表明, 以CA 为载体可以很好地固定 GOD 并保持其生物活性, 在无任何电子媒介体存在时, GOD在电极上实现了直接电子转移, GOD/CA/GC 电极 对葡萄糖具有很好的电催化性能. 关键词: 炭气凝胶; 葡萄糖氧化酶; 直接电化学 中图分类号: O646 Direct Electrochemistry and Immobilization of Glucose Oxidase on Nano-Carbon Aerogels YU Zhi-Hui1 SU Ting-Ting1 REN Cui-Hua1 LI Fan1 XIA Ding-Guo2,* CHENG Shui-Yuan1 1 ( College of Environmental and Energy Engineering, Beijing University of Technology, Beijing 100124, P. R. China; 2 College of Engineering, Peking University, Beijing 100871, P. R. China) Abstract: Nano-carbon aerogels (CA) were prepared by the reaction between resorcinol and formaldehyde in alkaline conditions. The morphology and microstructure of the CA was investigated by scanning electron microscopy and transmission electron microscopy. The specific area and the pore size distribution were calculated from the N2 ad