株蒜薹采后病原真菌的鉴定rDNAITS序列及碳源代谢.pdf

※生物工程 食品科学 2013, Vol.34, No.15 171 1株蒜薹采后病原真菌的鉴定、rDNA ITS序列及 碳源代谢指纹图谱分析 王友升，何欣萌，张 燕，张 萌，李 健，李丽萍 (北京工商大学食品学院，北京市食品风味化学重点实验室，食品添加剂与配料北京高校工程研究中心，北京 100048) 摘  要：为明确蒜薹贮藏期致病菌种类，增强采后病害防治针对性，从自然发病的苍山草薹上分离到1株丝状致病 真菌，经形态学特征观察及rDNA ITS序列分析确定为茄匍柄霉(Stemphylium solani)，分析其对95种碳源代谢指纹图 D α-D D 谱发现该菌对碳源适应性较强，最适碳源有 -果糖、蔗糖、麦芽糖、 -葡萄糖、 -半乳糖等75种，可利用碳源有 D D γ D N β D N D 5种： -葡萄糖胺、 -塔格糖、 -羟丁酸、 -苹果酸和尿苷，不可利用碳源有 -乙酰- - -甘露糖胺、 -乙酰- -半乳 L i 糖胺、 -海藻糖、-赤藓糖醇、葡糖醛酰胺等15种。 关键词：蒜薹；采后病害；ITS rDNA序列分析；碳源代谢指纹图谱 Identification, rDNA ITS Analysis and Carbon Metabolic Fingerprinting of a Pathogenic Fungus Isolated from Postharvest Garlic Sprout WANG You-sheng，HE Xin-meng，ZHANG Yan ，ZHANG Meng，LI Jian ，LI Li-ping (Beij ing Key Laboratory of Food Flavor Chemistry, Beij ing Higher Institution Engineering Research Center of Food Additives and Ingredients, School of Food and Chemical Engineering, Beij ing Technology and Business University, Beij ing 100048, China) Abstract ：A pathogenic fungus was isolated from infected garlic sprout during postharvest storage. Morphological observation and rDNA ITS analysis indicated that the isolated strain was Stemphylium solani. Metabolic fingerprinting investigation of 95 carbon sources suggested that the carbon metabolic fingerprinting of the strain was composed of 75 optimal carbon sources, including D-fructose, sucrose, maltose, α-D-glucose, D-galactose, and 5 available carbon sources (D-glucosamine, D-tagatose, hydroxybutyric acid, D-malic acid and uridine) as well as 15 unavailable carbon sources such as N-acetyl-D-galactosamine, N-acetyl-β-