微生物学美国IndianaUniversityPurdueUniversit网y授课14.pptVIP

Legionella BIOL 533 Lecture 14 History American Legion Convention in 1976 in Philadelphia hotel Respiratory distress and fever 200 affected 34 died History Six months after outbreak, CDC isolated bacteria from post-mortum lung tissue Inoculated lung tissue into peritoneal cavity of guinea pigs After animal became ill, removed its spleen and injected tissue into fertilized chicken eggs (rickettsial methodology) History Organism was thought to be unique, given new genus and species name: Legionella pneumophila Epidemiological search revealed outbreak in same hotel two years earlier Culture pr