长期低剂量摄入唑膦原药对大鼠毒性作用的研究.docVIP

长期低剂量摄入噻唑膦原药对大鼠毒性作用的研究 李本长，邓磊，周宁 (江西省职业病防治研究院 南昌 330006) [摘要] 目的 研究长期低剂量摄入噻唑膦原药对大鼠的毒性作用，确定其最大无作用剂量，为安全生产及慢性毒性实验提供剂量参考。方法 将80只SD大鼠（雌雄各半）按体重随机分为4组，分别为噻唑膦原药0.8mg/kg·bw·d组、4.0mg/ kg·bw·d组、20.0mg/ kg·bw·d和正常对照组。在实验结束后处死实验大鼠，同时检测血液学、血清生化、体重和脏器系数等指标，并对结果进行统计学处理。结果 高剂量组雄、雌性大鼠体质量增长缓慢，雄性大鼠高剂量组TG明显低于对照组，雌性大鼠高剂量组ALP明显高于对照组，雌性大鼠高剂量组TP、ALB、TBIL、CREA、GLU明显低于对照组，雌性大鼠低、中、高剂量组CHE明显低于对照组，且有剂量效应关系；雄性大鼠高剂量组脑、肺、肾、肾上腺、睾丸的脏器重量系数明显高于对照组，雌性大鼠高剂量组脑、肺、肾的脏器重量系数明显高于对照组，高剂量组的卵巢子宫的脏器重量系数明显低于对照组（p＜0.05，p＜0.01）。 结论 噻唑膦原药对雄、雌性大鼠经口染毒剂量为20.0mg/ kg·bw·d及以上时，对大鼠有毒性效应；长期低剂量摄入烯草酮原药对大鼠最大无作用剂量为4mg/kg·bw·d。 [关键词] 噻唑膦原药；长期；低剂量；除草剂 基金项目：江西省自然科学基金项目（20122BAB215026）作者单位：江西省职业病防治研究院；邮编：330006；邮箱：will1688@163.com作者简介：李本长（1983-），男，毒理学硕士，主治医师，研究方向为农药与神经毒理学The Toxicity study of long-term intake of 基金项目：江西省自然科学基金项目（20122BAB215026） 作者单位：江西省职业病防治研究院；邮编：330006；邮箱：will1688@163.com 作者简介：李本长（1983-），男，毒理学硕士，主治医师，研究方向为农药与神经毒理学 LI Ben-chang, DENG Lei, ZHOU Ning (JiangXi Institute of Occupational Disease Prevention, Nanchang, 330006) [Abstract] Objective To study the toxicity of rats that caused by long-term intake of low-dose Flumetsulam and determine the maximal non-effect of Flumetsulam, in order to provide the reference dose for safety in production and chronic toxicity experiment. Methods A total of 80 SD rats(half female and half male) were randomly divided into 4 groups, respectively, for the orginal drug 0.8mg/kg·bw·d group, 4.0mg/ kg·bw·d group,20.0mg/ kg·bw·d and normal control group based on body weight. Execute the rats meanwhile detect the indexes including hemology、biochemistry detection of serum、body weight and organ module after the end of the experiment, and the results were analyzed statistically. Results In the high dose group, the weight slow growth in male and female rats, the TG in high dose group of male rats and the TP、ALB、CREA、GLU in high dose group of female rats were lower than normal control group obviously, the ALP in high dose group of female rats were higher than normal control group, a