结果1共粹取了374份排遗样本成功标定237份样本的基因型63.pptVIP

Fecal DNA typing to determine the fine scale population structure and sex-biased dispersal pattern of Eurasian otter (Lutra lutra) in Kinmen CHUAN-CHIN HUANG1, SHOU-HSIEN LI2 AND LING-LING LEE1 1. Institute of Ecology and Evolutionary Biology, National Taiwan University, Taipei, Taiwan 106, ROC 2. Department of Life Science, National Taiwan Normal University, Taipei Taiwan 106, ROC According to previous studies, Eurasian otters are capable of moving long distance (Kruuk 1995). However, in a study on the spatial organization of Eurasian otters along 2 river systems in eastern Kinmen, Hung et al. (2004) found no individual moving across the 2 river systems within 1 year, even though these 2 river systems were only 2 km apart and had no topological barrier between them. Genetic information of 7 microsatellite loci also revealed that gene flow of otters between these 2 river systems was limited, and that the tendency of genetic differentiation was higher in females. Thus, we intended to examine the fine scale population structure of otters in the entire Kinmen and dispersal pattern of female versus male otters using noninvasive molecular method, i.e. examination of the genetic information of otters by genotyping DNA extracted from spraints collected from the entire Kinmen using 15 microsatellite markers (Huang et al., 2005). According to topography and distribution of inland waters, we divided Kinmen into 5 regions (NE, SE, NW, SW, M) and collected 362 fresh spraints of otters from 13 sampling sites in April and August 2003, and February 2004 (Fig. 1) We used 15 microsatellite markers and SRY primer in identification of individual genotype and sex typing. We used noninvasive molecular methods to investigate the fine scale population structure and to trace the movement pattern of Eurasian otters (Lutra Lutra) in Kinmen. We collected fresh spraints of otters from 13 sites in April and August 2003, and February 2004. DNA of 362 spraints was extracted and g