知母皂苷对自发性糖尿病GK大鼠降糖作用地实验研究.docVIP

知母皂苷对自发性糖尿病GK大鼠降糖作用的实验研究 王瑶基金来源：国家科技重大专项《重大新药创制》（课题号：2012Z001） 中国科学院上海药物研究所，上海 2012032 哈尔滨商业大学生命科学与环境科学研究中心，黑龙江哈尔滨 基金来源：国家科技重大专项《重大新药创制》（课题号：2012Z001） 中国科学院上海药物研究所，上海 201203 2 哈尔滨商业大学生命科学与环境科学研究中心，黑龙江哈尔滨 150076 ?.通讯作者：陈明苍，助理研究员 摘要 目的：探讨知母皂苷对自发性糖尿病Goto-Kakizaki（GK）大鼠的降血糖作用。方法：取雄性GK大鼠，根据GK大鼠血糖情况，随机分为模型对照组、阳性药物二甲双胍组、知母皂苷组；另设正常Wistar大鼠作为空白组。每周测定血糖、体重以及摄食量。并于实验结束时对所有大鼠进行OGTT试验。结果：知母皂苷不仅具有明显的降糖作用，并且能够减轻糖尿病大鼠口服葡萄糖后的血糖负荷。结论：知母皂苷能够改善糖尿病大鼠的糖耐量，具有一定的降血糖作用。 关键词：知母皂苷；GK大鼠；血糖；糖耐量 Anti-diabetic effects of timosaponin in spontaneous diabetic GK rats Objective: This study sought to evaluate the anti-diabetic effect of timosaponin in spontaneous diabetic GK rats. Methods: Male, diabetic GK rats were randomly divided into three groups: the timosaponin group, the metformin group and the model group according to the blood glucose. At the same time, the normal Wistar rats were used as the normal control group. The amount of blood glucose, food intake and body weight were measured every week. At the end of the experiment period, OGTT was performanced. Result: Timosaponin not only remarkably decreased the blood-glucose of diabetic rat, but also reduced glycemic-load in diabetic rats after oral glucose. Conclusion: Timosaponin improved the glucose tolerance and remarkably decreased the blood-glucose in spontaneous diabetic GK rats. Key words: timosaponin; GK rat; blood-glucose; the glucose tolerance GK大鼠是1973年由Goto及其同事通过Wistar近亲杂交而筛选出的非肥胖型自发性型糖尿病大鼠[参考文献： [] K. Srinivasan P. Ramarao. Animal models in type 2 diabetes reseach: An overview[J]. Indian J Med Res, 2007: 451-472.]。GK大鼠在其两周龄时出现稳定的高血糖，葡萄糖耐受量降低，并且出现2型糖尿病的早期并发症[[] A.Khan, S.M.Abdel-halim, et al. Abnormal insulin secretion and glucose metabolism in pancreatic islets from the spontaneously diabetic GK rat [J]. Diabetologia, 1993; 36: 3-8.,[] Portha B, Giroix M-H, Serrada p, Gangneurau M.-N, Movassat J, Rajas F, et al. beta-cell function and viability in the spontaneously diabetic GK rat: information from the GK