清醒大鼠室旁核微量注射心房钠尿肽对压力感受性反射敏感性.PDFVIP

生理学报 Acta Physiologica Sinica, April 25, 2005, 57 (2):175-180 175 研究论文 清醒大鼠室旁核微量注射心房钠尿肽对压力感受性反射 敏感性的影响 * 姜海英，金清华 ，李迎军，许东元，金元哲，金秀吉 延边大学医学院生理学教研室，延吉133000 摘 要：心房钠尿肽(atrial natriuretic peptide, ANP)作为一种神经递质或调质可能参与心血管活动的中枢调节。本实验在清 醒大鼠室旁核(paraventricular nucleus, PVN)注射ANP ，探讨其对压力感受性反射敏感性的影响，并通过侧脑室注射血管升 压素受体Ⅰ阻断剂OPC-21268 ，观察ANP 对压力感受性反射敏感性的调节是否与中枢血管升压素有关。实验中观察到，在 PVN 内微量注射ANP (6 、60 ng/0.2 µ l) 可明显提高压力感受性反射敏感性(P 0.05) ，侧脑室预先注射OPC-21268 (0.45 µg/3 µl)后，ANP 对压力感受性反射敏感性的增强作用明显减弱(P0.05) 。静脉注射ANP (60 ng/0.04 ml)不影响压力感 受性反射敏感性。上述结果提示，心房钠尿肽对压力感受性反射活动起易化作用，心房钠尿肽的这种中枢作用可能部分 通过中枢血管升压素介导。 关键词：心房钠尿肽；室旁核；压力感受性反射；血管升压素 中图分类号：Q463; R338.2 Effect of microinjection of atrial natriuretic peptide into the paraventricular nucleus on baroreflex sensitivity in conscious rats * JIANG Hai-Ying, JIN Qing-Hua , LI Ying-Jun, XU Dong-Yuan, JIN Yuan-Zhe, JIN Xiu-Ji Department of Physiology, Yanbian University Medical School, Yanji 133000, China Abstract: The role of atrial natriuretic peptide (ANP) in the central regulation of the circulation is known to be a neurotransmitter or a neuromodulator, but its actions on baroreceptor reflex function are not fully resolved. The present study examined the role of ANP (6, 60 ng/0.2 µl) by direct microinjection into the hypothalamic paraventricular nucleus (PVN) in conscious rats. OPC-21268 (0.45 µg/ 3 µl), an antagonist of the V1 receptor, was microinjected into the lateral ventricle to examine whether the effect of ANP on baroreflex sensitivity is mediated by vasopressin (VP). ANP significantly increased the baroreflex sensitivity, and OPC-21268 attenuated the increase of baroreflex sensitivity induced by ANP. Intravenous injections of ANP (60 ng/0.04 ml) did not affect baroreflex sensitivity. These results suggest that ANP in the PVN may produce a facilitative effect on baroreflex, and the effect may be via,