等离子体聚丙烯酸薄膜的制备.doc

PAGE PAGE 1 等离子体聚丙烯酸薄膜的制备及固定白蛋白以提高抗凝血性能? ?基金项目：博士点基金(2009018412001)、中央高校项目基金(SWJTU12CX014) 作者简介：景凤娟（1973），女，副教授，离子束表面改性、生物材料E-mail：fengjuanj@ 景凤娟，王进，黄楠 （西南交通大学材料先进技术教育部重点实验室，四川省成都市 邮编610031） 摘要：本文通过等离子体反应装置，采用丙烯酸单体在纯钛表面制备含有羧基的等离子体聚合丙烯酸薄膜，并在其表面固定白蛋白，对其抗凝血性能进行了研究。通过FTIR、XPS、接触角测试、表面轮廓仪、羧基定量实验、溶血实验、血小板粘附实验对其表面性能进行了评价。结果显示，等离子体聚丙烯酸薄膜出现了羧基官能团；等离子体聚丙烯酸薄膜及其表面固定白蛋白后，具有很小的溶血率；在等离子体聚丙烯酸薄膜上固定白蛋白时，pH为4.0时的固定量多于pH为7.0时的固定量；等离子体聚丙烯酸薄膜固定白蛋白后，血小板的粘附数量、变形与聚集行为有明显的改善。 关键词：材料表面与界面；等离子体聚丙烯酸薄膜；羧基；血小板粘附行为；抗凝血；白蛋白 中图分类号：R318.08 TB34 文献标识码： 文章编号：201212-1127 The immobilization of albumin on plasma polymeric acrylic acid films and the resulting effects on hemocompatibility JING Fengjuan, WANG Jing, HUANG Nan ( Key Lab. Of Advanced Materials Technology, Ministry of Education, Southwest Jiaotong University, Chengdu 610031,China) Abstract：The plasma-polymerized acrylic acid films containing of carboxyl group were produced on titanium substrates by the acrylic acid and argon plasma treatments. Bovine serum albumin was immobilized on the optimized plasma-polymerized acrylic acid films. FTIR, XPS, contact angle test, carboxyl group quantification test, blood platelet adhesion, hemolysis were used to evaluate the properties of materials. The carboxyl groups were observed on the films of deposited acrylic acid films. The BSA can be immobilized effectively on the plasma-polymerized acrylic acid films at pH 4.0 compared to pH at 7.0. The adhesion, denaturing and aggregation of the platelets were impeded significantly on albumin immobilized on the plasma-polymerized acrylic acid films compared to titanium films. The plasma-polymerized acrylic acid films showed low hemolytic ratio. Key words：suface and interface of materials; plasma-polymerized acrylic acid films; carboxyl group; platelet adhesion; anticoagulation; albumin 引言 血栓是与血液接触的生物医学装置和人工器官临床使用中存在的主要问题。通过在这些材料与器械表面固定抗凝血的生物大分子白蛋白，对其进行表面生物化改性，可以更好地改善其表面的抗凝血性能。然而无机材料表面只有很少或几乎没有羧基、羟基或胺基官能团的存在，这为后续的生物分子固定带来了困难。因此，如何在无机薄膜表面构建官能团，为后续的生物分