A catabolic pathway for the degradation of chrysene by Pseudoxanthomonas sp. PNK-04英文学习资料 .pdfVIP

R E S E A R C H L E T T E R A catabolic pathway for the degradation of chrysene by Pseudoxanthomonas sp. PNK-04 Anand S. Nayak, Sanjeev Kumar Sanganal, Santosh Kumar Mudde, Anjaneya Oblesha Timmanagouda B. Karegoudar Department of Biochemistry, Gulbarga University, Karnataka, India Correspondence: T. B. Karegoudar, Abstract Department of Biochemistry, Gulbarga University, Gulbarga 585106, Karnataka, The chrysene-degrading bacterium Pseudoxanthomonas sp. PNK-04 was isolated India. Tel.: 191 8472 263 289; fax: 191 8472 from a coal sample. Three novel metabolites, hydroxyphenanthroic acid, 1- 245 632; e-mail: goudartbk@ hydroxy-2-naphthoic acid and salicylic acid, were identified by TLC, HPLC and MS. Key enzyme activities, namely 1-hydroxy-2-naphthoate hydroxylase, 1,2- Received 10 January 2011; revised 11 April dihydroxynaphthalene dioxygenase, salicylaldehyde dehydrogenase and catechol- 2011; accepted 28 April 2011. 1,2-dioxygenase, were noted in the cell-free extract. These results suggest that Final version published online 31 May 2011. chrysene is catabolized via hydroxyphenanthroic acid, 1-hydroxy-2-naphthoic acid, salicylic acid and catechol. The terminal aromatic metabolite, catechol, is DOI:10.1111/j.1574-6968.2011.02301.x then catabolized by catechol-1,2-dioxygenase to cis,cis-muconic acid, ultimately