大孔树脂纯化革皮氏海参总皂苷工艺-食品科学.pdf

※工艺技术 食品科学 2011, Vol. 32, No. 12 1 于林芳，王 超，王玉明，王 林，董 平，薛 勇 * ，薛长湖 ( 中国海洋大学食品科学与工程学院，山东 青岛 266003) 比较7 种大孔吸附树脂对革皮氏海参总皂苷的静态吸附和解吸效果，从中筛选出适合该海参总皂苷分离纯 化的树脂，并对其动态吸附和解吸性能进行研究。结果表明，XAD761 树脂最适合革皮氏海参总皂苷的纯化。当 上样液质量浓度 2mg/mL ，流速0.8mL/min ，上样液体积9BV 时，树脂达到动态吸附饱和。再用 5BV 70% 乙醇， 以 1mL/min 的流速可以洗脱完全，解吸率为93.31% 。经过树脂的纯化，皂苷的纯度由原来的21% 提高到 65% 。 海参；皂苷；分离纯化；大孔树脂 Macroporous Resin Purification of Saponins from the Sea Cucumber Pearsonothria graeffei YU Lin-fang ，WANG Chao ，WANG Yu-ming ，WANG Lin ，DONG Ping ，XUE Yong* ，XUE Chang-hu (College of Food Science and Technology, Ocean University of China, Qingdao 266003, China) Abstract ：The adsorption and desorption rates of 7 types of macroporous resins towards total saponins from the sea cucumber Pearsonothria graeffei were investigated. Among the studied macroporous resins, XAD761 resin was the most suitable to purify crude total flavonoid extract from Pearsonothria graeffei . When 9 BV of 2 mg/mL sample was flowed through XAD761 resin column at a speed of 0.8 mL/min, the adsorption reached dynamic equilibrium. The adsorbed saponins could be completely eluted with 5 BV of 70% ( V/V) ethanol at 1 mL/min, with a desorption rate of 93.31%. After the purification, the purity of total saponins was increased from 21% to 65%. Key words ：sea cucumber ；saponin ；purification ；macroporous resin 中图分类号：R284.2 文献标识码：A 文章编号：1002-6630(2011)12-0001-04 海参属棘皮动物门海参纲，自古以来被认为是一种 孔吸附树脂纯化海参皂苷无需耗用有机试剂，树脂可重 名贵的滋补食品和药材。海参皂苷是海参的主要次生代 复使用，方法经济简便，适用于规模化工业生产。董 谢产物，也是其进行化学防御的物质基础。它们具有 平等[15]对几种市售海参中总皂苷的含量进行了研究，结 抗真菌[ 1 ] [ 2 ] [ 3 ] [ 4 ] 果发现革皮氏海参(Pearsonothuria graeffei)总皂苷的含量 、抗肿瘤 、细胞毒 、溶血 等多种生物活 性。海参皂苷的分离纯化过程很复杂，常规的步