灰分校正对范氏洗涤法同时测定烟草中ADF和ADL的影响-烟草科技.pdf

2015 年2 月 烟草科技 Feb. 2015 第48 卷第2 期 Tobacco Science Technology Vol. 48 No. 2 灰分校正对范氏洗涤法同时测定烟草中 ADF和ADL的影响 张 婕，葛永辉，向章敏，周淑平，杨 慧，耿召良* 贵州省烟草科学研究院，贵阳市观山湖区龙潭坝路29 号 550081 摘要：为实现烟草中酸性洗涤纤维（ADF ）和酸洗木质素（ADL ）的同时测定，利用范氏洗涤法在 ADL 分析步骤中可得到未经灰分校正的ADF 测定值这一特点，研究了灰分校正对不同类型烟 草样品中ADF 测定结果的影响及ADF 和ADL 中残余硅酸盐量的相互关系。结果表明：① ADF 中硅酸盐量与样品中的灰分及ADL 中硅酸盐量均极显著正相关。②对除烤烟外的部分 高灰分量类型烟草样品，ADF 中的硅酸盐残余量会对结果造成过高的正偏差，但利用ADL 中 硅酸盐的量对未经灰分校正的ADF 值进行校正，即可使不同类型烟草样品中ADF 和纤维素分 析的相对偏差控制在5% 以内，无需对ADF 进行灰化破坏，实现了ADF 和ADL 的同时分析，极 大地提高了分析效率。 关键词：烟草；范氏洗涤法；灰分校正；酸性洗涤纤维（ADF ）；酸洗木质素（ADL ）；同时测定 中图分类号：TS411.1 文献标志码：B 文章编号：1002-0861（2015 ）02-0058-05 DOI: 10.16135/j.issn 1002-0861 InfluencesofAshCorrectiononSimultaneousDeterminationofADFandADL inTobaccobyVanSoestMethod ZHANG Jie, GE Yonghui, XIANG Zhangmin, ZHOU Shuping, YANG Hui, and GENG Zhaoliang* Guizhou Tobacco Science Research Institute, Guiyang 550081, China Abstract: In order to determine the acid detergent fiber (ADF) and acid detergent lignin (ADL) in tobacco simultaneously, and taking account of that the ADF value inclusive of ash could be obtained during the ADL analyzing process of Van Soest method, the influences of ash correction on the measurement of ADF in tobacco samples of different types were investigated and the relationship between the residual silicate contents in ADF and ADL was studied. The results showed that: 1) The silicate content in ADF extremely significantly positively correlated to the ash content in samples and the silicate content in ADL. 2) For tobacco samples of high ash content, excluding flue-cured tobacco, the residual silicate would create bigger positive deviations in ADF determination. However, a relative deviation of less than 5% could be