酶解法制备可口革囊星虫抗菌物质的研究-中国抗生素杂志.pdfVIP

中国抗生素杂志2018年4月第43卷第4期 . 471 . 文章编号：1001-8689(2018)04-0471-05 酶解法制备可口革囊星虫抗菌物质的研究 * 许翔 蔡怀依 袁爽 李勇勇 娄永江 宁梦华 (宁波大学海洋学院，宁波3152 11) 摘要：目的 确定可口革囊星虫体壁中抗菌物质的最佳酶解工艺。方法 以可口革囊星虫体壁为原料，利用筛选出的木瓜 蛋白酶酶解可口革囊星虫体壁蛋白，获得含可口革囊星虫体壁的酶解产物，并用抑菌圈法比较不同条件下酶解产物对大肠埃希 菌活性的影响。在单因素试验的基础上，应用四因素三水平正交设计分析法，探讨酶解温度、pH值、酶解时间、加酶量及底物 浓度对酶解产物抑菌活性的影响。结论 木瓜蛋白酶最适合水解可口革囊星虫体壁制备抗菌物质，其最佳工艺条件为：底物浓 度为4g/L 、加酶量为4000U/g、温度为55℃、pH值为8.0 的条件下酶解4h ，获得平均抑菌圈大小为(15.78±0.5)mm 。 关键词：抗菌物质；酶解法；可口革囊星虫；木瓜蛋白酶 中图分类号：S967.5 文献标志码：A Research on enzymatic hydrolysis production of the antibacterial substances from Phasolosma esculenta Xu Xiang, Cai Huai-yi, Yuan Shuang, Li Yong-yong, Lou Yong-jiang and Ning Meng-hua (School of Marine Science, Ningbo University, Ningbo 3152 11) Abstract Objective To determine the optimum enzyme reaction conditions for producing the antibacterial substances in the wall of Phasolosma esculenta . Methods As the basic material, the integument of Phasolosma esculenta is enzymolyzed by the selected papaya protease to obtain the antimicrobial substances in the integument of phasolosmaesculenta and the effects of enzymolyzed substances on the activity of Escherichia coli . On the basis of the single factor test, four factors and three levels of orthogonal design were used to study the effects of the enzymolysis temperature, pH values, the enzymolysis time, the enzyme dosage and substrate concentrations on the antibacterial activity of the hydrolyzates. Conclusion Papaya protease is best for enzymolyzing antimicrobial substances in the integument of phasolosmaes