止血带下克林霉素在兔肌肉组织中的分布特性.pdfVIP

止血带下克林霉素在兔肌肉组织中的分布特性 叶冬梅，孙可，陈晓宇(广西壮族自治区人民医院，南宁 530021) 摘要：目的 建立高效液相色谱法测定兔肌肉组织中的克林霉素，并对止血带下肌肉组织中克林霉素的分布特征进行研 究。方法 色谱条件为乙腈-四氢呋喃-磷酸盐缓冲液(30 ∶1 ∶70 ，pH=5) 为流动相；柱温：40 ℃；流速：0.8 mL·min1 ； 替硝唑为内标，Luna C18 柱(250 mm×4.6 mm ，5 μm)进行分离，检测波长为205 nm 。测定静脉注射克林霉素30 ，60 ，90 ， 120 min 后上止血带分别阻断血液循环30 ，60 ，90 min 实验组与未止血对照组的肌肉组织中药物含量。结果 克林霉素 在 10.58~264.5 μg·mL1 内线性关系良好，日内RSD ≤2.58% ，日间RSD ≤4.20% 。实验组克林霉素含量与对照组比较， P0.01 ；相同止血时机，不同止血时间药物含量比较，P0.05 ；不同止血时机(30 ，60 ，90 ，120 min)药物含量比较，P0.01 ； 给药后60 min 上止血带阻断肢体血液循环，克林霉素在肌肉组织的含量最高，为39.30 µg·g1。结论 方法准确可靠、简 便、重复性好；实验组克林霉素含量与对照组有显著性差异；围手术期预防感染，上止血带时机是影响克林霉素组织分 布的主要因素，给药后60 min 止血，药物可充分分布到肌肉软组织中，提高预防感染效果。 关键词：克林霉素；止血带；高效液相色谱法；分布 中图分类号：R965.1; R973.1 文献标志码：A 文章编号：1007-7693(2010)07-0584-04 Distribution of Clindamycin in Muscle of Rabbit with Tourniquet YE Dongmei, SUN Ke, CHEN Xiaoyu(People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China) ABSTRACT: OBJECTIVE To establish an HPLC method to determine the content of clindamycin in muscle of rabbits, and to study its characteristics of distribution in muscle tissue of rabbits which were stopped bleeding by tourniquet. METHODS The mobile phase consisted of a mixture of acetonitrile-tetrahydrofuran-phosphoric acid(30 ∶1 ∶70, pH=5) with a flow rate of 0.8 mL·min1. Tinidazole was used as an internal standard, the analytical column was packed with 5 μm C18 at 40 ℃, and the detection wavelength was 205 nm. Experiment groups were stopped bleeding for 30, 60, 90 min after drug administration at 30, 60, 90, 120 min, respectively. Then, clindamycin in muscle of exeriment and control groups were determined by HPLC. RESULTS The calibration curve of clindamycin was linear in the range of 10.58264.5 μg·mL1. The RSD of intra and inter-day were sm