循环肿瘤细胞.pptxVIP

循环肿瘤细胞 （Circulating tumor cells(CTCs)）;目录;概念;概念;研究意义;研究难点;; 非特异性富集方法;CTCs的富集;CTCs细胞大小富集方法;Scanning electron microscopy (SEM) images of: (A) commercial track-etched membranes. The random distribution of pores results in the formation of larger holes by fusion of neighboring holes. (B) A microfabricated hole in a parylene membrane filter. (C) Captured cells on a parylene membrane with controlled distribution and size of holes. (D) SEM view of a captured CTC.;Schematic of a pool-dam chip. The decreasing width of gaps between successive pools allows the separation of CTCs in the first pool, followed by WBCs and RBCs in the subsequent pools ;Density-based enrichment utilizes Ficoll (or similar density gradient medium) to enrich for mononuclear cells (including CTCs) from other blood components. ;The DEP/G-FFF principle for cell separation. Different cells are levitated at different heights determined by the collective effect of DEP and gravity (FDEPz and Fgrav). After cells reach their equilibriums, a parabolic laminar flow having different velocity profiles at different heights (VFFF2 N VFFF1) collects the cells at the same output but at different times. Cells within thefaster profile (VFFF2) are collected first, followed by the cells lie within the slower profile (VFFF1).;Immunomagnetic separation involves the use of iron-conjugated antibodies targeted toward CTCs (e.g., EpCAM; positive selection) or contaminating blood cells (e.g., CD45; negative selection) and incubation in a magnetic field.;CTCs微流体技术富集法;CTCs各种富集方法的优缺点;CTCs的检测;CTCs部分检测方法;;CTC的研发趋势;;CTC的研发趋势;CtDNA as an alternative to CTC analysis;CTC与CTDNA的分析比较;CTC的研发趋势;CTC的研发趋势;CTC的功能研究;Thanks!