苦木化学成分及质量研究.pdf

羟基-β-咔巴啉，1-甲酸-β-咔巴啉，1-丙酸基-β-咔巴啉，3- 甲基-铁屎米-5,6-二酮，4- 甲氧基-5-羟基铁屎米酮，1-甲氧甲酰基-β-咔巴啉，4,5-二甲氧基-铁屎米酮）的含量测 定方法，并用此法考察了不同产地、不同生长年限及不同药用部位的苦木质量。结果 表明，不同产地苦木的生物碱类成分含量差异大，生长年限长的苦木中生物碱成分含 量高，苦木茎中的生物碱类成分含量高于叶、枝中的含量。 关键词： 苦木；化学成分；生物碱；含量测定；指纹图谱 IV 万方数据 Studies on Chemical Constituents and Quality of Picrasma quassioides Speciality: Chinese Materia Medica Author: Lai Zhengquan Tutor: Research fellow Lin Ji ABSTRACT The thesis deals with the study on Picrasma quassioides (D.Dom)Benn., including the chemical constituents research, the HPLC fingerprint and quantitative analysis studies based on literature review. The aim is to establish quality standard and control the quality of this medical materials effectively. Chemical research of Picrasma quassioides (D.Dom)Benn. The isolation was with the intention of producing the alkaloids reference substances with high contents and strong pharmacological activities based on present literature review and preliminary HPLC-MS study on 85% ethanol extract of stem of Picrasma quassioides (D.Dom)Benn.. Nineteen compounds were obtained by integrated techniques including silica gel, etc. Nine ones of them were ascertained by physicochemical properties and modern spectroscopic analysis including UV, 1H-NMR, 13C-NMR, EI-MS and so on. They respectively are 4,5-Dimethoxycanthin-6-one (compound 3) ，1-Methoxycarbony-β-carboline (compound 5),3-Methylcanthin-5,6-dione (compound 6), 3-Methylcanthin-5,6-dione (compound 7), β-Carbolin-1-yl 4,8-Dimethoxy-β-carboline-1-1ylethyl Ketone (compound 12), 4–Methoxy-5- hydro -xylcanthin-6-one