甘露寡糖纳米制剂的制备及抗病促生长作用解析研究.docVIP

PAGE / NUMPAGES 甘露寡糖纳M制剂的制备及抗病促生长作用研究 摘 要 本实验目的是探究制备甘露寡糖的方法，研究与开发甘露寡糖脂质体纳M制剂，评价甘露寡糖脂质体纳M制剂对动物抗病促生长和增强免疫力的功效。 实验采用枯草芽孢杆菌诱生的β-甘露聚糖酶水解魔芋精粉来制备甘露寡糖；选用不同的脂质体制备方法来制备甘露寡糖脂质体纳M制剂；观察了甘露寡糖脂质体纳M制剂对正常小鼠脾淋巴细胞体外增殖和血清中的免疫指标、免疫器官指数的影响；研究了甘露寡糖脂质体纳M制剂对鸡的生长发育和免疫功能的影响；研究了甘露寡糖脂质体纳M制剂对鸡白痢沙门氏菌的预防效果；观察了甘露寡糖脂质体纳M制剂对鸡球虫致病力的作用效果。 结果表明：枯草芽孢杆菌不是高产β-甘露聚糖酶的菌株，诱生出的β-甘露聚糖酶活性很低，不适合用于甘露寡糖的制备。实验筛选出乙醇注入法来制备甘露寡糖脂质体纳M制剂，正交实验证明，制备100 mL甘露寡糖脂质体纳M制剂的最佳参数为大豆卵磷脂800 mg，胆固醇200 mg，无水乙醇8 mL，磷酸盐缓冲液的pH 7.5，甘露寡糖1000 mg，其包封率高达54.47%，粒径分布在200 nm左右。甘露寡糖纳M制剂能够显著提高小鼠的胸腺指数，降低血清中IL-2的水平。甘露寡糖脂质体纳M制剂能够提高感染沙门氏菌鸡的保护率，减少鸡的死亡率。甘露寡糖纳M制剂能提高正常鸡的增重率，增加采食量。甘露寡糖脂质体纳M制剂能降低感染鸡球虫鸡的盲肠病变值和卵囊值，但不宜作为抗球虫药应用。 结论：已成功制备出甘露寡糖脂质体纳M制剂，其性质稳定，粒径达到纳M级，它能调节动物机体的免疫功能，改善动物肠道微生态平衡，促进动物的生长发育，提高机体的抗病力。 关键词 甘露寡糖 脂质体 纳M制剂 STUDY ON PREPARATION OF MOS NANO PREPARATION AND ITS EFFECTS ON GROWTH AND RESISTANCE TO DISEASES ABSTRACT The aim of this study is to investigate MOS preparation and developMOS nano liposome preparation.The effects of MOS liposomenano formulations on growth, resistance to diseases and immune functions were observed. In this study, Bacillus subtiliswas induced by the β-mannanase hydrolysis of konjac flour to produce the MOS。 Different methods were used to prepare nano-MOS liposome preparation。 The effects of MOS nano liposome preparation on normal murine spleen lymphocyte proliferation, serum immune parameters and indices of immune organs were investigated。 The effects of the MOS nano liposome preparation on growth and immune function in chicken were evaluated。 The action of the MOS nano liposome preparation against infection of Salmonella pullorum was observed。 The effect of MOS nano liposome preparation on infection of Eimeria tenella was assessed. The results showed thatBacillus subtilis is not a high β-mannanase strain induced yield of β-mannanase activity, of which was very low and not suitable for the preparation of oligosaccharides. Screening experiments were carried out to find a suitable method prepare MOS nano liposome pr