发光酶基因luxAB标记硅酸盐细菌NBT菌株的研究.pdfVIP

2004 7 15 7 CHIN ESE JO UR NAL OF A PPL IED ECOLO GY, Jul. 2004, 15( 7)124 1~ 1244 lux AB NBT * * * 何琳燕 黄为一 ( , 210095) . 025% . BT 200 g ml- 1 N BT-R 200 , luxA B pT R 102 lux AB pRK 20 13 , NBT . , , 670 10- 5, 111 . RL 85 , , 20 3 , . 100 1- 9332( 2004) 07 - 1241- 04 Q93 A Introduction of bioluminescence genes into silicate-dissolving bacteria strain NBT. HE L nyan , HU AN G W e y (Dep artment of M icrobiology, Nanj ing Agricultural University, Nanj ing 2 10095, China) . Chin. J. App l. Ecol . , 2004 , 15( 7) : 1241~ 1244 . In th s study, s l cate- d ssolv ng bacter a NBT stra n w as g row n w th 0 . 25% maltose as carbon source, and the r- f amp c n- res stance w as g ener ated by ultrav olet mutagenes s and str eak natural zed to 200 gml- 1 . T hrough tr- parental cross, the luxAB genes w ere ntroduced nto N BT- R200 at the help of p RK2013. T he lum nescence ac- t v ty of the hybr d stra n w as tested , w h ch nd cated that all colon es had a h g h lum nescence act v ty and kanamyc n- , tetr acyct ne-and r famp c n- res stance. T he NBT- R200 cells prepared from n t al logar t hm c growt h phage w ere mo re l kely to be sens t v e to fore g n DNA , and the max mum translo cat on frequency w as up to 6 . 70 10- 5 . In add t on , the opt mal mat ng r at o w as 111. T he potass um release ab l ty from f eldspar and the lu- m nescence of lux AB genes marked s l cate- d ssolv ng bacter a R L85 stra n w er e stable, and hence, the RL85 w as ava lable to r h zosphere m croecology r esearches. Key words S