非小细胞肺癌血浆EGFR突变与肿瘤标志物水平的关系.docVIP

非小细胞肺癌血浆EGFR突变与肿瘤标志物水平的关系 肖 何，王 阁，王 东，杨镇洲，杨宇馨 (400042重庆，第三军医大学大坪医院野战外科研究所肿瘤中心) [摘要] 目的 探讨非小细胞肺癌外周血游离DNA样本EGFR突变率与临床基线特征和血清肿瘤标志物表达水平的关系。方法 收集本院确诊为非小细胞肺癌166例。利用PCR和变性高效液相色谱法分析EGFR19和21外显子突变。其中89例同时利用多肿瘤标志物蛋白芯片检测系统检测癌胚抗原(CEA)、糖类抗原125(CA125)、糖类抗原153(CA153)等血清肿瘤标志物，分析突变率与临床基线特征和肿瘤标志物表达水平的关系。结果 166例非小细胞肺癌中，EGFR总突变率19.9%。女性突率显著高于男性(P=0.037)。腺癌突变率高于其他组织学类型，但未达到显著水平(P=0.096)。CEA、CA153中高表达患者EGFR突变率显著高于低表达者(P=0.048，P=0.017)，CA199高表达患者仅有高于中低表达者的趋势(P=0.093)。多变量Logistic分析表明，腺癌、CA153、CEA中高表达与突变率相关。结论 对于晚期非小细胞肺癌，血浆EGFR突变率与肿瘤标志物表达水平明显相关。血清标志物水平可作为进一步判断EGFR突变优势群体的因素。 [关键词] 非小细胞肺癌；表皮生长因子；血浆游离DNA；突变；肿瘤标志物 [中图法分类号] [文献标志码] A The relationship between EGFR mutations detected in plasma and expressions of serum tumor markers in patients with non-small cell lung cancer Xiao He, Wang Ge, Wang Dong, Yang Zhenzhou, Yang Yuxin (Cancer Center, Daping Hospital and Research Institute of Surgery, Third Military Medical University, Chongqing, 400042, China) [Abstract] Objective To investigate the relationship between EGFR mutations rate detected in plasma free DNA and clinical characteristics of baseline as well as expression level of serum tumor markers in patients with advanced non-small cell lung cancer (NSCLC). Method One hundred and sixty six patients with non-small cell lung cancer confirmed pathologically were studied. PCR and DHPLC were performed to detect EGFR mutations in exons 19 and 21 in 166 cases with NSCLC. The serum levels of 12 tumor makers were analyzed with multi-tumor markers protein biochip detective system in 89 patients simultaneously. The associations between EGFR mutation rate and the patients’ clinical characteristics at baseline along with the corresponding serum level of tumor makers were determined. Results Mutation rate of EGFR was 19.9% in 166 NSCLC patients. Mutation rate in female was significantly higher than that in male patients (P=0.037). A nonsignificant trend for higher mutation rate in adenocarcinoma was observed compared wit