硒化壳寡糖地合成及抗氧化性研究.docVIP

实用文档 实用文档 文案大全 文案大全 摘 要 【目的】以亚硒酸钠和壳寡糖为原料，合成硒化壳寡糖，并对其进行表征以及对其抗氧化活性性进行研究。 【方法】硒化壳寡糖的合成，采取化学方法，以亚硒酸钠和壳寡糖为原料进行。通过紫外及红外对合成的硒化壳寡糖进行表征。硒化壳寡糖的抗氧化活性通过邻二氮菲-氧化法，邻苯三酚自氧化法，以及总抗氧化能力试剂盒进行测定。 【结果】成功制备了硒化壳寡糖产品，紫外和红外光谱结果表明，合成的硒化壳寡糖产品成功在壳寡糖中引入了硒；两种方法测的硒化壳寡糖中硒含量分别为9.02mg/ml和8.82mg/ml；对壳寡糖和硒化壳寡糖的抗氧化能力测定结果显示壳寡糖和硒化壳寡糖具有明显的抗氧化能力且硒化壳寡糖抗氧化能力高于壳寡糖。 【结论】本实验证实以亚硒酸钠和壳寡糖能够成功合成硒化壳寡糖，且合成的硒化壳寡糖比壳寡糖具有更好的抗氧化能力，为研究低毒性、有效的有机补硒产品提供了思路。 关键词：硒化壳寡糖；硒含量；抗氧化 Study on Antioxidant Activity of Chitooligosaccharide -Se4+ Abstract 【Objective】 We use sodium selenite and Chitooligosaccharide as raw materials to synthesis Chitooligosaccharide-Se4+ and study it on its characterization and antioxidant activity. 【Method】Sodium selenite and Chitooligosaccharide are used to synthesis Chitooligosaccharide- Se4+ in this experiment by chemical method. It was characterizde by ultraviolet and infrared method. Its antioxidant activity was studied by phenanthroline - Fe oxidation, pyrogallol autoxidation,and T-AOC kit. 【Results】The UV spectrum and FTIR showed that the synthesis Chitooligosaccharide- Se4+ is successful. The two methods showed that the content of Selenium in Chitooligosaccharide- Se4+ were 9.02mg/ml and 8.82mg/ml.The results of study on antioxidant activity of Chitooligosaccharide-Se4+ showed that both Chitooligosaccharide and Chitooligosaccharide -Se4+ have antioxidant activity and Chitooligosaccharide-Se4+ presented higher antioxidant activity than Chitooligosaccharide. 【Conclusion】This expetiment presented that Chitooligosaccharide-Se4+ can be prepared successfully ,and whats more it has higher antioxidant activity than Chitooligosaccharide.This can lead us to study more low toxicity and effective products witch can provide the idea of organicselenium. Keywords:Chitooligosaccharide-Se4+;Selenium content ;Antioxidant activity 目 录 TOC \o 1-3 \h \z \u 摘 要 I Abstract II 引 言 - 1 - 1 综述 - 2 - 1.1壳寡糖简介 - 2 - 1.1.1壳寡糖的理化性质 - 2 - 1.1.2壳寡糖的主要功能 - 2 - 1.1.3壳寡糖的应用 - 3 - 1.