生物大分子的分离和纯化.ppt

* * Pump * Gradient Mixer GM-1, Mixer 6.0 ml, and Mixer 0.6 ml. * Monitor-UV * ?KTA?explorer is the first chromatography system from the new ?KTAdesign platform. This system is intended for preparative laboratory chromatography, in particular method development, and has been designed to meet the needs of scientists in industry working in a regulated environment, according to GLP or GMP requirements. ?KTAexplorer uses UNICORN? control system software allowing easy scale up to production. IQ/OQ documentation packages are available on request for ?KTAexplorer. Each package consists of printed forms, detailed test protocols and expected results. Also included are descriptions of the software program and hardware components for UNICORN, which are necessary for validation of the control system. The BioProcess System offers process scale solutions to the pharmaceutical industry. The systems are used for clinical trials through to full scale production. The majority of systems are customer designed to meet the specific needs of the application. They are controlled by UNICORN control system designed for GMP production. Validation Support and IQ/OQ Documentation Packages are available. Standard process systems are available in 3 mm, 6 mm and 10 mm configurations. The design of those standard systems can vary according to demand. Contact the Engineering Dept. for further details. * Monitor-UV * Monitor-UV * Monitor-UV * Mini protein III * 标准的层析设备 * * 梯度混合仪 泵 分部收集仪 层析柱 * 紫外检测器 记录仪 * 自动化层析设备 1 * * * * 条条大路通罗马！ 蛋白质的纯化无固定的模式，不同的蛋白质有不同的纯化方法，同一种蛋白质也可采取不同的纯化方法，但最终得到的蛋白质其稳定性和纯度也有所差别，这就涉及到操作者的实践经验及对纯化艺术的理解。 * 四、几种与蛋白质纯化相关的技术 蛋白质浓缩 电泳 蛋白浓度测定 * （一）蛋白质浓缩 透析袋浓缩法 冷冻干燥浓缩法 超滤浓缩法 凝胶浓缩法 浓缩胶浓缩法 * （二）电 泳 SDS－聚丙烯酰胺凝胶电泳 等电聚焦电泳 双向电泳 * 蛋白质在高于或低于其pI的溶液中为带电的颗粒，在电场中能向正极或负极移动。这种通过蛋白质在电场中泳动而达到分离各种蛋白质的技术, 称为电泳(elctrophoresis) 。 根据支撑物的不同，可分为薄膜电泳、凝胶电泳等。 * SDS-聚丙烯酰胺凝胶电泳，常用于蛋白质分子量的测定。 等电聚焦电泳，通过蛋白质等电点的差异而分离蛋白质的电泳方法。 双向凝胶电泳，是蛋白质组学研究的重要技术。 * 双向电泳 * * * * （三）蛋白质浓度的测定 紫外法：蛋白质的芳香族氨基酸在280nm 有吸收。操作和设备要求