真核细胞DNA提取和酶切鉴定.pptVIP

* Weigh out 0.5 g of agarose into a 250 mL conical flask. Add 50 mL of 0.5xTBE , swirl to mix. It is good to use a large container, as long as it fits in the microwave, because the agarose boils over easily. * * Microwave for about 1 minute to dissolve the agarose. The agarose solution can boil over very easily so keep checking it. It is good to stop it after 45 seconds and give it a swirl. It can become superheated and NOT boil until you take it out whereupon it boils out all over you hands. So wear gloves and hold it at arms length. You can use a bunsen burner instead of a microwave - ju