原花青素对肠缺血再灌注大鼠胃肠动力的保护作用.DOCVIP

原花青素对肠缺血再灌注大鼠肠道平滑肌运动功能的保护作用 李禹呈2，杨拯1，孙金菊2，杨海燕2，罗兰2，宋强1，林伟1 （610500成都，成都医学院：基础医学实验教学中心1，临床医学院2） [摘要] 目的 探究原花青素对肠缺血再灌注(Ischemia-Reperfusion, I/R) 损伤大鼠肠道平滑肌的保护作用及机制。 方法 21只3～5月龄SD大鼠，体质量（200±20）g，随机分为假手术组 (n=7)，缺血再灌注组 (n=7)，原花青素组 (n=7)，原花青素组给予原花青素(100mg/kg/day) 预处理，其余两组灌胃等量生理盐水，连续给药5天。采用夹闭肠系膜上动脉(Superior Mesenteric Artery, SMA) 1 h后再灌注6 h建立肠I/R损伤大鼠模型，随后打开腹腔取回肠组织测定平滑肌收缩力，腹主动脉取血离心后，测定血清超氧化物歧化酶(SOD)活力值和一氧化氮(NO)含量。 结果 与假手术组比较，肠I/R后肠道平滑肌收缩活力明显减弱(P0.01)，血清SOD活力降低(P0.05)，并且NO含量增多(P0.05)；原花青素预处理的大鼠与肠缺血再灌注组大鼠比较，肠道平滑肌收缩活力和血清SOD活力显著提高(P0.05)，NO的含量明显减少(P0.05)。 结论 原花青素可通过提高SOD活力，减少血清中NO含量来改善肠I/R后的肠道平滑肌运动功能。 [关键词] 原花青素；肠缺血再灌注；平滑肌收缩力 Protective effects of proanthocyanidins on intestinal smooth muscle in rats after intestinal-ischemia reperfusion injury Li yucheng2, Yang zheng1, Sun jinju2, Yang Haiyan2, Luo Lan2, Song qiang 1, Lin wei1 (Chengdu Medical College, Chengdu 610500, China: Department of Clinical Medicine1, Department of Experiment and Education Centre2) [Abstract] Objective Aim to investigate the protective effects and possible mechanism of proanthocyanidins on intestinal smooth muscle contractility of rats after ischemia reperfusion (I/R). Methods 21 SD rats were randomly divided into sham group (n=7), intestinal I/R group (n=7), and proanthocyanidins group (n=7). Proanthocyanidins group was administrated with proanthocyanidins as pretreatment constantly for 5 days, and the other two groups were administrated with the same dose of physiological saline constantly for 5 days. The intestinal I/R model was induced by occlusion of superior mesenteric artery of rat for 1 h followed by a period of reperfusion for 6 h. Following reperfusion, abdominal cavity was opened and ileum tissue was taken to test smooth muscle motility, and blood was taken from abdominal aorta to be centrifuged to get serum to determine SOD activity and the content of NO. Results Compared with sham group, the intestinal smooth muscle contractility of I/R group decreased significantly (P0.01) after I/R