动物转基因克隆的分子生物学基础.ppt

李宁 转基因动物研究的里程碑 转基因动物的制作技术 原核期胚胎的显微注射 逆转录病毒载体注射MII期的卵母细胞 精子头与外源DNA合并注射卵母细胞 体细胞核移植技术制作转基因动物 逆转录病毒载体感染发育早期的动物胚胎 原始生殖细胞技术 精子载体方法 胚胎干细胞技术 利用转基因动物制药的途径 血液、尿腺 、乳腺、禽卵、昆虫 Strategy for Production of Human Medicine by Transgenic Animals 转基因奶牛和细胞培养技术生产抗体蛋白的成本比较 人乳与牛乳主要成分的比较 人奶与牛奶的功能差异及其优缺点 Personal Ballistic Protection: “superior energy-to-break”  mechanical properties with weight 25%  lighter than aramid-based fibers “生物钢”的应用领域与发展需求 ?国防用“生物钢”-新型生物材料的生产 ?医用人工韧带、关节等生物材料的生产 ?高附加值特殊用途-粘性蛋白等的生产 转基因奶羊和细胞培养技术生产抗体蛋白的成本比较 The Comparison between Different Fermentation Technology PPL Therapeutics plc 生产人营养保健（医疗）品 ！ Expansion of the Founder Transgenics Comparison of Milk Componentbetween Human and Cow Problems Presented by Cow-Milk Feeding Infants or Human Medical Function of Human Lactoferrin Bacteriostatic effect on micro-organisms Bacteriolytic effect on broad spectrum of bacteria Growth stimulating effect on bifidus strains Iron transport protein Zinc transport protein Trophic effect on mucosal cells Possible Application of Human Lactoferrin as Therapeutical 影响转基因动物产业化的根本原因 Inducible expression system Cre-LoxP system Animal Transgenics 国内外研究现状与趋势 研究方案和内容 ?化学去核法以使体细胞克隆操作简便、提高工作规模和克隆效率 研究方案和内容 Animal Transgenics Immunological Barriers 治疗型克隆技术将更具有挑战性和广泛应用前景？ Production of Biosteel by Tg Goat The strongest materials in the World Personal Ballistic Protection: “superior energy-to-break”  mechanical properties with weight 25%  lighter than aramid-based fibers SPIDER MAN- USA ARMY ? USA Biosteel Production Basement 诊断、治疗人类疾病及新药筛选的转基因动物模型 基因过量的表达 基因敲除(Knock-out)或敲入(Knock-in) 病毒和其他病原的DNA插入诱变 318 knock-out-in mice 47 over-expression mice 15 virus-induced mice BIOPURE: a cinderala technology Biopure Headquarter in Boston ANIMAL CLONING ES CELL TECHNOLOGY Mice were cloned in a large sc