DNAWorks全基因合成介绍.pptVIP

Gene Synthesis using DNAWorks Dr. David Hoover Helix Systems, SCB, CIT, NIH Gene Synthesis Several methods ligation - incredibly tedious and inefficient FokI - sequence dependent (type IIs r.e.) serial cloning - sequence dependent assembly or self-priming PCR Gene Synthesis Methods Protein Expression Protein/Structure Independent Factors: promoters and upstream elements translational initiation and termination mRNA stability codon bias Protein/Structure Dependent Factors: folding and aggregation proteolysis and degradation secretion and localization Codon Bias Synthetic Genes Benefits: Codon