分子部分(全)最新[1]..ppt

生化与分子生物学实验 概述 实验设计： Get to know the protein you want to clone Acquire the coding sequence of the protein Decide the vector you want to use Design primers for coding sequence PCR Prepare PCR templates PCR and prepare insertion fragment (digestion ) Prepare vector fragments (digestion, purification) Ligation, transformation, screening, verification Transfer expression plasmid to expression host cells Expression, purification, characterization of cloned protein 生化与分子生物学实验I 实验用试剂的配制. 器皿的准备及灭菌 实验内容（以下内容均按每小组4人计算） 1）每小组共配制 100ml为固体培养基， 装在250ml锥型瓶中； 高压灭菌（121℃/20min) 然后4℃放置备用。 LB固、液体培养基配方（按1L计算