小桐子SRAP-PCR体系优化和M1代变异.PDFVIP

2009, 23( 2) : 209~ 2 13 209 Journal of NuclearAgricultural Sciences : 1000- 8551( 2009) 02- 209- 05 SRAP-PCR M1 1, 2 1 3 2 陈敦萍 李 凌 沈世华 杨 清 ( 11 , 4007 15; 21 , 666303; 31 , 100095) 60 : 利用SRAP 分子标记手段对筛选出的18 株经化学试剂Co C辐照和低能离子注入等诱变处 后的小桐子变异植株进行遗传多样性分析结果显示, 22 对SRAP 引物一共扩增到266 条带, 其中54 条为多态性条带, 多态性比例为2013% ; 选出的18 株形态变异的小桐子植株, 大部分不仅在外部形态上 发生了变异, 而且在DNA 上也发生了基因突变这为今后利用诱变技术培育高产优质抗逆性强的小桐 子新品种奠定了实验基础 : 小桐子; 诱变; 变异; SRAP OPTIMIZATION OF SRAP-PCR SYSTEM AND M MUTANT MOLECULAR 1 IDENTIFICATION OF M IN Jatrop ha curcas L. 1 1, 2 1 3 2 CHEN Dun- p ng LI L ng SHEN Sh- hua YANG Q ng ( 11Southwest University , the College of Horticulture and LandscapeArchitecture, ChongQing 400716; 21Xishuangbanna TropicalBotanic Gardens , the ChineseAcade y of Sciences , YunNan, Xishuangbanna 666303; 31Institute of Botany , the ChineseAcade y of Sciences , BeiJing 100095) 60 Abstract: 18 var ant plants from mutagenes s of chem cal reagents, Co C- rrad at on and low energy on mplantat on were used for SRAP markers to dent