毛细管电泳02知识课件.pptVIP

Using higher running buffer concentration can also improve the shape of peaks, also ascribed to stacking effect. Effect of the buffer ionic strength on peak shape and migration time of peptides. Buffer: sodium phosphate, pH 2.44; 30 kV; 20 °C; 200 nm; 57 (50) cm ′ 75 mm capillary. Peak identification: (1) dynorphin; (2) bradykinin; (3) angiotensin II; (4) TRH; (5) LHRH; (6) bombesin; (7) leu-enkephalin; (8) met-enkephalin; (9) oxytocin. From McLaughlin et al., Beckman Technical Information Bulletin TIBC-106 (1991). Effect of stacking in peak enhancement. Pinching to avoiding flooding. Gati