水产动物育种学 第七章 细胞融合与核移植讲解材料.pptVIP

Removal of the nucleus surrounded by a small amount of ooplasm from a mouse germinal vesicle oocyte. Takeuchi T et al. Hum. Reprod. 1999;14:1312-1317 ? European Society of Human Reproduction and Embryology Oocyte in the process of nuclear transfer * 然后用发圈拨动去核卵，使胚盘朝上，再把已吸有细胞核的吸管对准此胚盘的近中央处剌入，并把细胞核（连同核外微量细胞质一起）注入胚盘内。 A grafted oocyte (couplet of a karyoplast and cytoplast) aligned between two microelectrodes. Takeuchi T et al. Hum. Reprod. 1999;14:1312-1317 ? European Society of Human Reproduction and Embryology 核移植操作系统 鱼类核移植杂种细胞由于卵外胶膜被破坏，需在等渗的Holtfreter液中培养一段时间，然后逐渐转入低渗溶液，早期胚胎发育过后可入曝气水中