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Confocal Microscopy Confocal Microscopy: Basic Concepts Confocal microscopy offers several advantages over conventional optical microscopy, including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens. In the biomedical sciences, a major application of confocal microscopy involves imaging either fixed or living cells and tissues that have usually been labeled with one or more fluorescent probes. When fluorescent specimens are imaged using a conventional widefield optical microscope, secondary fluorescence emitted b