Protein A, Protein G, and Protein 基本原理及应用.pdfVIP

Chapter 24 Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L Remko Griep and John McDougall 24.1 Introduction Today, monoclonal antibodies (mAbs) form the largest category of biopharmaceu- ticals in clinical trials, and their number is expanding rapidly (DataMonitor 2007a, b). The antibodies or functional antibody fragments are being produced not only in artificial production systems such as mammalian cells, yeast, bacteria, and plant cells but also in transgenic animals such as goats, sheep, and cows. Regardless of the